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pPB_EF1a_HA-tNGFR_PuroR

Plasmid

#212652

Purpose

piggyBac vector to express HA-tNGFR with puromycin resistance

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

tNGFR

Use

Piggybac

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_FLAG-tNGFR_PuroR

Plasmid

#212651

Purpose

piggyBac vector to express FLAG-tNGFR with puromycin resistance

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

tNGFR

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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ABEmax-Puro V2

Plasmid

#226955

Purpose

ABEmax plasmid enabling A:T to G:C base editing using a single plasmid.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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DG SaCas9 puro V3

Plasmid

#226960

Purpose

CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 7 deletion

Plasmid

#224388

Purpose

Lenti plasmid for generating GNB1L _WD 7 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

WD 7 domain deleted (aa 286-323)

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion

Plasmid

#224387

Purpose

Lenti plasmid for generating GNB1L _WD 6 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

WD 6 domain deleted (aa 242-282)

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 4 deletion

Plasmid

#224385

Purpose

Lenti plasmid for generating GNB1L _WD 4 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

WD 4 domain deleted (aa 153-195)

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-MDA5delta2CARD

Plasmid

#174225

Purpose

Expresses human MDA5 that lacks 2 CARD domains

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MDA5 (IFIH1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

deletion of 2CARD

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1loop

Plasmid

#215032

Purpose

Mammalian expression of human TWIST1 (with NEUROD1 loop)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop (aa134-141, IIPTLPSD) replaced w…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROG2loop

Plasmid

#215033

Purpose

Mammalian expression of human TWIST1 (with NEUROG2 loop)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop (aa137-141, TLPSD) replaced with…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1ins

Plasmid

#215036

Purpose

Mammalian expression of human TWIST1 (with insertion from NEUROD1)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop with insertion from NEUROD1 bHLH…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROG2ins

Plasmid

#215037

Purpose

Mammalian expression of human TWIST1 (with insertion from NEUROG2)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop with insertion from NEUROG2 bHLH…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1L

Plasmid

#215043

Purpose

Mammalian expression of human TWIST1 (with fragment of loop from NEUROD1)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Left portion of TWIST1 bHLH loop (aa137-139, TLP)…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1R

Plasmid

#215044

Purpose

Mammalian expression of human TWIST1 (with fragment of loop from NEUROD1)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Right portion of TWIST1 bHLH loop (aa139-141, PSD…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1M

Plasmid

#215045

Purpose

Mammalian expression of human TWIST1 (with fragment of loop from NEUROD1)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Middle portion of TWIST1 bHLH loop (aa139, P) rep…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperior.retro.puro-sh-γ-tubulin

Plasmid

#205602

Purpose

Tet-inducible expression of shRNA targeting gamma-tubulin in human cells, retroviral

Depositor

Tarun Kapoor

Article

Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

Insert

sh-g-Tubulin

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBabepuro-TGFA

Plasmid

#189610

Purpose

Retroviral expression vector for TGFA

Depositor

Thierry Tchenio

Insert

TGFA (TGFA Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

SV40 early promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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sgVRK2-puro

Plasmid

#199636

Purpose

sgRNA guide against VRK2

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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sgLacZ-puro

Plasmid

#199635

Purpose

sgRNA control guide targeting bacterial LacZ

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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sgCTRL-puro

Plasmid

#199634

Purpose

sgRNA control guide targeting intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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