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NAX_CAGGS_CAS9_Hygro

Plasmid

#167859

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Nan Cher Yeo, Alejandro Chavez

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBAC-ECFP-15xQUAS_TATA-mcd8-GFP-SV40

Plasmid

#104878

Purpose

piggyBac vector of 15xQUAS-mCD8:GFP; used for mosquito tranformation

Depositor

Christopher Potter

Article

Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

Insert

mCD8:GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEPIC1.1

Plasmid

#169897

Purpose

Empty constitutive expression vector with piggyBac, IRES, and mClover3

Depositor

Richard Schneider

Article

Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

Type

Empty backbone

Use

Avian expression

Tags

3x FLAG 2x StrepII

Expression

Mammalian

Mutation

Promoter

mouse PGK

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pPB: PGK-H2B-mIFP-T2A-rTetR-GBP-polyA-zeo

Plasmid

#163084

Purpose

rTetR DNA binding domain fused to GBP (anti-GFP nanobody)

Depositor

Lacra Bintu

Article

Van et al Nat Commun. 2021 Jan 22;12(1):537. doi:

Insert

anti-GFP nanobody

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_GFP*_Intein_DD

Plasmid

#162812

Purpose

STELLA plasmid for amber suppression of C-terminally labelled GFP

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

GFP(C-TAG)-Intein-DD

Use

Piggybac

Tags

Expression

Mammalian

Mutation

GFP(Opal247,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*NoBody

Plasmid

#162807

Purpose

STELLA plasmid for amber suppression of N-terminally labelled NoBody

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)NoBody

Use

Piggybac

Tags

Expression

Mammalian

Mutation

NoBody (Met1, Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_EBFP2

Plasmid

#167857

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Nan Cher Yeo, Alejandro Chavez

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_EYFP

Plasmid

#167858

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Nan Cher Yeo, Alejandro Chavez

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW

Plasmid

#133368

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_EYFP

Plasmid

#167890

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Nan Cher Yeo, Alejandro Chavez

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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BII-ChPtinGW

Plasmid

#133377

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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BII-ChPtW

Plasmid

#133379

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BII-CBtW

Plasmid

#133372

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050S (UASt-dCasRx)

Plasmid

#132419

Purpose

Express dCasRx under UASt promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Inserts

CasRx
UASt+hsp70

Use

Tags

Expression

Insect

Mutation

Promoter

UASt

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

Plasmid

#162810

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_Intein_DD

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*GFP

Plasmid

#162806

Purpose

STELLA plasmid for amber suppression of N-terminally labelled GFP

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

EF1_Ub-(N-TAG)GFP

Use

Piggybac

Tags

Expression

Mammalian

Mutation

GFP (Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Opie2-PuroR[traF]

Plasmid

#131615

Purpose

Expresses a functional PuroR only in females and dsRed in both genders.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Insert

PuroR[traF]

Use

Tags

Expression

Insect

Mutation

Promoter

Opie2

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pTBL2368 MTK PB puro backbone

Plasmid

#226673

Purpose

Empty MTK PB cargo backbone marked with puromycin resistance.

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-SOD1

Plasmid

#222568

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SOD1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SOD1 (SOD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-RAD51

Plasmid

#222567

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of RAD51

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

RAD51 (RAD51 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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