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pIBA2-Int-SpyTag

Plasmid

#198038

Purpose

Expression plasmid for producing SpyTagged stalled secretion variant of intimin

Depositor

Jack Leo

Article

Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

Insert

eaeA

Use

Tags

SpyTag

Expression

Bacterial

Mutation

lacks native signal peptide (amino acids 1-39)

Promoter

tet

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pIBA2-IntHA453-SpyTag

Plasmid

#198039

Purpose

Expression plasmid for producing SpyTagged intimin

Depositor

Jack Leo

Article

Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

Insert

eaeA

Use

Tags

SpyTag

Expression

Bacterial

Mutation

double HA tag inserted at position 453; lacks nat…

Promoter

tet

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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PE-SUMO CRYGS Q16C

Plasmid

#217674

Purpose

For expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experiment

Depositor

Larry David

Insert

CRYGS (CRYGS Human)

Use

Tags

6XHis and SUMO

Expression

Bacterial

Mutation

Glutamine 16 changed to Cysteine

Promoter

T7

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pESUMO-hRORg2

Plasmid

#170329

Purpose

Expresses RORg isoform 2 with N-terminal HisSUMO tag in bacteria

Depositor

Patrick Griffin

Article

Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

Insert

RORG isoform 2 (RORC Human)

Use

Tags

HisSUMO

Expression

Bacterial

Mutation

codon optimized

Promoter

T7

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pESUMO-hRORg1

Plasmid

#170330

Purpose

Expresses RORg isoform 1 with N-terminal HisSUMO tag in bacteria

Depositor

Patrick Griffin

Article

Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

Insert

RORG isoform 1 (RORC Human)

Use

Tags

HisSUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-Stx3-L77A/L80A

Plasmid

#100273

Purpose

Expresses GST-Stx3-L77A/L80A in bacteria.

Depositor

Thomas Weimbs

Article

Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

Insert

Stx3-1-265-L77A/L80A (Stx3 Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

No transmembrane domain; residues 4-264 present, …

Promoter

Tac

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-Stx3-E78A/E83A

Plasmid

#100272

Purpose

Expresses GST-Stx3-E78A/E83A in bacteria.

Depositor

Thomas Weimbs

Article

Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

Insert

Stx3-1-265-E78A/E83A (Stx3 Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

No transmembrane domain; residues 4-264 present, …

Promoter

Tac

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-Stx3-LE165/166AA

Plasmid

#100271

Purpose

Expresses GST-tagged open-conformation mutant of Stx3 in bacteria.

Depositor

Thomas Weimbs

Article

Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

Insert

Stx3-1-265-LE165/166AA (Stx3 Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

No transmembrane domain; residues 4-264 present, …

Promoter

tac

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pASK-mSAN1-Streptag

Plasmid

#117165

Purpose

Expresses Strep-tagged murine SAN1 nuclease in bacteria

Depositor

Ian Macara

Article

Andrews et al Nat Commun. 2018 Jul 3;9(1):2592. doi: 1

Insert

mSAN1 (Fam120b Mouse)

Use

Tags

Streptag

Expression

Bacterial

Mutation

Promoter

tetR/tetA

Available since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pPN094

Plasmid

#91621

Purpose

Express sgRNA targeting human GRIN2A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

GRIN2A (GRIN2A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN095

Plasmid

#91622

Purpose

Express sgRNA targeting human GRIN2A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

GRIN2A (GRIN2A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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