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Plasmid#167634PurposeExpress FepA D12C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA D12C
UseTagsExpressionBacterialMutationFepA with D12C mutation. (Numbering based on star…PromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 Nxf1B
Plasmid#170266PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing full length Nxf1BDepositorArticleInsertnuclear export factor 1, B6 allele
UseTagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastMutationPromoterCUP1Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 Nxf1C
Plasmid#170267PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing full length Nxf1CDepositorArticleInsertnuclear export factor 1, CAST allele
UseTagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastMutationPromoterCUP1Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS29
Plasmid#171205Purposebacterial expression for HIS purification of MEG-3 with mutations in the HMG-like motifDepositorInsertmeg-3 (meg-3 Nematode)
UseTags6xHISExpressionBacterialMutationF705A,Y708A,Y713A, N725APromoterAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
lvl0 TagRFP CTAG
Plasmid#153401PurposeRed fluorescent protein CTAG codon optimised for Arabidopsis thalianaDepositorInsertTagRFP CTAG
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
lvl0 MethylAblet
Plasmid#153412PurposeIt is used to bypass the Level 1 cloning and directly feed terminators into Level 2DepositorInsertamilCP
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
lvl0 fluc CDS
Plasmid#153405PurposeFirefly luciferase coding sequenceDepositorInsertfirefly luciferase CDS
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
lvl0 YPet CTAG
Plasmid#153402PurposeYellow fluorescent protein CTAG codon optimised for Arabidopsis thalianaDepositorInsertYpet CTAG
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
lvl0 eYFP CTAG
Plasmid#153396PurposeYellow fluorescent protein CTAG codon optimised for Arabidopsis thalianaDepositorInserteYFP CTAG
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW005
Plasmid#131806PurposeVector with aTc inducible M. laminosus Fd1 with a C-terminal fused mRFP1DepositorArticleInsertMastigocladus laminosus ferredoxin
UseSynthetic BiologyTagsmRFP1ExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW002
Plasmid#131591PurposeVector with aTc inducible M. laminosus Fd1 without BbsI restriction sites in the vector backboneDepositorArticleInsertMastigocladus laminosus ferredoxin
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO-M7-puro
Plasmid#131697Purposeexpresses MHV68 M7 promoter driving puromycin resistanceDepositorArticleInsertM7-puro (GAMMAHV.M7 MHV68)
UseRetroviralTagsExpressionMammalianMutationPromoterM7Available sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG07-pLac-NRII_L16R
Plasmid#102464PurposeExpresses NRII protein with L16R mutation in E. coli. The expression is controlled by a pLac promoter.DepositorArticleInsertNRII (ntrB )
UseTagsExpressionBacterialMutationLeucine 16 to ArgininePromoterpLacAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSG08-pLac-NRII_H139N
Plasmid#102465PurposeExpresses NRII protein with H139N mutation in E. coli. The expression is controlled by a pLac promoter.DepositorArticleInsertNRII (ntrB )
UseTagsExpressionBacterialMutationHistidine 139 to AsparaginePromoterpLacAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-D-yckABD
Plasmid#112790PurposeDownstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilisDepositorArticleInsertyckABD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBUE411-GG
Plasmid#198232PurposeWheat GRF4-GIF1 chimera in pBUE411DepositorInsertGRF4-GIF1 chimera cassette
UseCRISPR; Plant binary vectorTagsExpressionPlantMutationPromoterUbiquitinAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cre-MS2_UbC-GFP (Cre_GFP)
Plasmid#219536Purposea. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotorDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Cys-3HB-SPYcatcher
Plasmid#214838PurposeThis plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-3HB-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPYtag-Histag-Avidin
Plasmid#214836PurposeThis plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-Histag-Avidin
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Non tagged Avidin
Plasmid#214835PurposeThis plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertNon tagged Avidin
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only