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Plasmid#31585DepositorInsertcoatomer protein complex, subunit epsilon (COPE Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP-VPR
Plasmid#183936PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFPDepositorInserttdPCP-GFP-VPR
UseTagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-QCP-3xBFP
Plasmid#158206PurposeQb coat protein fused to three repeats of the blue fluorescent protein under a Efla promoter.DepositorInsertQCP under the Elfa promoter and three BFP tags
UseSynthetic BiologyTags3xBFPExpressionMammalianMutationPromoterElfaAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
MCP-mCherry-TIS11B
Plasmid#207670PurposeTo express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIS11BDepositorInsertTIS11B (ZFP36L1 Human)
UseTagsMCP-mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYI006
Plasmid#170840PurposeFRO6p::GUSPlus::tOCS in pFP100DepositorInsertFRO6p::GUSPlus::tOCS
UseTagsExpressionPlantMutationPromoterFRO6Available sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
CTAG_eGFP-GHtag-HDEL
Plasmid#136096PurposeFP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tagDepositorInsertCTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
XXXXA-pHG165c
Plasmid#170110PurposeConstitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.DepositorInsertXXXXA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
XXXXA_Q182A_R287H-pHG165c
Plasmid#170111PurposeConstitutively expressed , mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.DepositorInsertXXXXA_Q182A_R278H
UseTagsExpressionBacterialMutationQ182A, R287HPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xQb oligo-library based
Plasmid#158201PurposeCassette of 10 different binding sites with high affinity to QB phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of QB based on oligo library experiment
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRWJB006
Plasmid#160113PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoter35SAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRWJB004
Plasmid#160112PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoterUBQ10Available sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-vdh
Plasmid#128134PurposeThe cell-free adapted backbone, pBEAST, expressing vdh (the enzyme converting benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertvdh
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK287
Plasmid#71120PurposeProduces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (H6UctB) in E. coliDepositorInsertformyl-CoA:oxalate CoA-transferase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCP-Luc (MCP-1 promoter in pGL3-basic)
Plasmid#40324DepositorInsertMCP-1 promoter (Ccl2 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationcontains MCP-1 promoter region through the nucleo…PromoterMCP-1Available sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2CP-VPg(FCV)
Plasmid#167314PurposeTemplate pDNA to prepare CaVT mRNADepositorInsertCaVT
UseSynthetic BiologyTagsDYK-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_PCP-T5Exo
Plasmid#214736Purposeencodes T5 exonuclease with a PP7 coat protein fused to the N terminusDepositorInsertCAG_PCP-T5Exo_bGH
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
StrKDEL-IRES_mScarleti_Sec23A
Plasmid#117273PurposeExpresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23ADepositorInsertsUseTagsmScarlet-iExpressionMammalianMutationHuman Sec23A; Sequencing showed a mutation in Sec…PromoterCMV and IRESAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG MCP-VP64
Plasmid#92357PurposeCAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.DepositorInsertMCP-VP64
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRW004-SM-destination-proUBQ10-Cas9
Plasmid#104438PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoterUBQ10Available sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-NbMS2
Plasmid#171701PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the coat protein of bacteriophage MS2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only