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Plasmid#168803PurposePiggyBac transposon construct with doxycycline inducible NEUROD1 + GFP expressionDepositorInsertNEUROD1 (NEUROD1 Human, Synthetic)
UseSynthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-1
Plasmid#72343PurposeMammalian expression of secreted mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBPExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKMD106e - intein-Cas9(S219)
Plasmid#64190PurposeExpresses intein-Cas9(S219) in mammalian cellsDepositorInsertmammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG
UseCRISPRTagsNLS - 3x FLAG (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-6
Plasmid#72346PurposeMammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBPExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AU1-mTOR-L1460P
Plasmid#19995DepositorInsertmammalian target of rapamycin-L1460P (Mtor Rat)
UseTagsAU1ExpressionMammalianMutationChanged Leucine 1460 to ProlinePromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-rnc-sfGFP
Plasmid#51560PurposeGene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).DepositorInsertrnc (rnc E. coli)
UseTagsGLESTCRHASLAVLADERRFSA and superfolder GFPExpressionBacterialMutationContains additonal aa at the end of the sfGFP in …PromoterAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKMD106h - intein-Cas9(C574)
Plasmid#64191PurposeExpresses intein-Cas9(C574) in mammalian cellsDepositorInsertmammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG
UseCRISPRTagsNLS - 3x FLAG (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMamCol1
Plasmid#215184PurposeAllows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coliDepositorInserttsPurple
UseAAV, CRISPR, and Synthetic Biology ; Recombinant …TagsSNAC-StrepII tagExpressionBacterial and MammalianMutationPromoterTetON 3G Bidirectional for Mammalian and Trc with…Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-8
Plasmid#72347PurposeMammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…ExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB_iETV2_P2A_GFP_Puro
Plasmid#168805PurposePiggyBac transposon construct with doxycycline inducible ETV2 + GFP expressionDepositorInsertETV2 (ETV2 Human, Synthetic)
UseSynthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-11
Plasmid#72349PurposeMammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…ExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe RFP1-Smad2 neo
Plasmid#58491Purposemammalian expression of mRFP1-tagged mouse Smad2DepositorInsertSmad2 (Smad2 Mouse)
UseTagsmRFP1ExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-3
Plasmid#72345PurposeMammalian expression of secreted N-terminally 8His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…ExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-2
Plasmid#72344PurposeMammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBPExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKMD111e - intein-Cas9(S219-G521R)
Plasmid#64192PurposeExpresses intein-Cas9(S219-G521R) in mammalian cellsDepositorInsertmammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG
UseCRISPRTagsNLS - 3x FLAG (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-M3(T3D)471-721
Plasmid#56657PurposeGFP reporter for cytoplasmic inclusions formed by the fused, C-terminal region of protein muNS from mammalian orthoreovirus Type 3 DearingDepositorInsertM3(T3D)421-721
UseTagsEGFPExpressionMammalianMutationinsert encodes aa 471-721 + native stop codon of …PromoterCMV-IEAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAS_FLAG-Mx1201 PylRS
Plasmid#154764Purposeplasmid with Mx1201 PylRS for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertMx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
UseTagsFLAGExpressionMammalianMutationPromoterEF1Available sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-10
Plasmid#72348PurposeMammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…ExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUT8-Dox
Plasmid#124631PurposeDoxycycline-inducible FUT8 expression in mammalian cellsDepositorInsertsAlpha-(1,6)-fucosyltransferase
Tetracycline repressor A3 mutant
TagFP635
UseTagsExpressionMammalianMutationPromoterTRE-tight and hEF1aAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT_FLAG-Mx PylRS YA
Plasmid#140012PurposePlasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
UseTagsFLAGExpressionMammalianMutationY126APromoterEF1Available sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only