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pEX-SP-CFP-STIM1(23-685)

Plasmid

#18858

Depositor

Tobias Meyer

Article

Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

Insert

Stromal interaction molecule 1 (STIM1 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted between residues 1-23 (signal peptid…

Promoter

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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FRB-ECFP(W66A)-Giantin

Plasmid

#67903

Purpose

Expresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van_Unen et al Scientific Reports 5, Article number: 14

Insert

FRB-ECFP(W66A)-Giantin

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pH2B-SCFP1-2A-mTq2-NES

Plasmid

#60491

Purpose

Co-expresses two cyan fluorescent protein variants, a short fluorescence lifetime CFP variant in the nucleus and a CFP variant with a long lifetime (mTurquoise2) in the cytoplasm

Depositor

Dorus Gadella

Article

Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.

Inserts

H2B-SCFP1
mTurquoise2-NES

Use

Tags

SCFP1 and mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pCFP-LacI-HOXD13wt-IDR

Plasmid

#145255

Purpose

Expresses fusion of ECFP-LacI and HOXD13wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13wt-IDR (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRetro-XT-PAK2-CFP(puro)-WT

Plasmid

#81176

Purpose

Retroviral vector for expression of wild-type PAK2 with a CFP fusion

Depositor

Angeliki Malliri

Article

Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

Insert

p21 (RAC1) activated kinase 2 (PAK2 Human)

Use

Retroviral

Tags

CFP

Expression

Mutation

Promoter

P-tight TRE

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
KN206: pMVP/eCFP-DEST

Plasmid

#121854

Purpose

pMVP destination vector, empty expression vector backbone w/ eCFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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5HT6-CFP-Venus(H148G)

Plasmid

#47501

Purpose

Fluorescent reporter for pH inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

Venus(H148G)

Use

Tags

5HT6 and CFP

Expression

Mammalian

Mutation

H148G mutation

Promoter

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
optGluCl beta Y182F ECFP

Plasmid

#15105

Depositor

Henry Lester

Article

Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

Insert

GluCl beta (glc-2 Nematode)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Codon optimized cDNA, Y182F

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

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P4U2C8T2-v1-1 (Pl-tetO1-deCFP) (135p)

Plasmid

#70211

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deCFP

Use

Tags

Expression

Mutation

Promoter

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pEX-CFP-STIM1-deltaK

Plasmid

#18860

Depositor

Tobias Meyer

Article

Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

Insert

STIM1 (STIM1 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Stop codon at amino acid position 671 of STIM1. C…

Promoter

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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TIA1 RRM-CFP-FRBx5

Plasmid

#103780

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-CFP-FRBx5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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CFP-CitA6-130-Venus

Plasmid

#128016

Purpose

Unmodified FLIP CitA6-130 sensor

Depositor

Uwe Sauer

Article

Ewald et al PLoS One. 2011;6(12):e28245. doi: 10.137

Insert

CitA

Use

Tags

Expression

Mutation

see Depositor Comments

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Lyn-CFP-GAI(1-172)

Plasmid

#37313

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Use

Tags

CFP and Lyn

Expression

Mammalian

Mutation

contains aa 1-172

Promoter

CMV

Available since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
pLCMV:ECFP(loxP)(FRT)HNF1B

Plasmid

#31439

Depositor

Gerhart Ryffel

Article

Sauert et al PLoS One. 2012;7(3):e33522. Epub 2012 Ma

Insert

HNF1B (HNF1B Human)

Use

Cre/Lox; Flp/frt

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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LCMV:ECFP(loxP)(FRT)MCS

Plasmid

#31377

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

ECFP(loxP)MCS

Use

Cre/Lox; Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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Lyn-CFP-GAI(1-151)

Plasmid

#37312

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Use

Tags

CFP and Lyn

Expression

Mammalian

Mutation

contains aa 1-151

Promoter

CMV

Available since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
KN302: pMVP/SB/eCFP-DEST

Plasmid

#121863

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eCFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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Lyn-CFP-GAI(1-532)

Plasmid

#37314

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Use

Tags

CFP and Lyn

Expression

Mammalian

Mutation

contains aa 1-532

Promoter

CMV

Available since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
V956 pLPS-3'ECFP - Acceptor

Plasmid

#11698

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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LB801: pMVP/PB/eCFP-DEST

Plasmid

#121872

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ eCFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge