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pCW-Cas9-Blast
Plasmid#83481PurposeLentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminaase -T2A -rtTADepositorInsertsSpCas9
Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
UseCRISPR and LentiviralTagsExpressionMammalianMutationReplaced puromycin N-acetyltransferase on the ori…PromoterTight Tre and human phosphoglycerate kinase 1 pro…Available sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-G-Flamp2
Plasmid#192782Purposegreen biosensor for cAMP in mammalian cellsDepositorInsertG-Flamp2
UseTagsHis tagExpressionMammalianMutationPromoterCAGAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-p300
Plasmid#23252DepositorInsertp300 (EP300 Human)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-Sec16A
Plasmid#36155PurposeExpresses Sec16A as a GFP fusionDepositorInsertSec16A (SEC16A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
RINS1
Plasmid#107290PurposePlasmid for the expression of the RINS1 sensorDepositorInsertRINS1
UseTagssfGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-GFP-ECL-3TM-tevs-KKMP V3
Plasmid#179640PurposeTEVP-inducible membrane-bound GFP RELEASE constructDepositorInsertMembrane-bound GFP RELEASE
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
RpHLuorin2
Plasmid#180227Purposefor bacterial expression of recombinant ratiometric pHluorin2DepositorInsertRatiometric pHLuorin2
UseTagsHis-tag followed by thrombin cleavage siteExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_VSVG-SBP-EGFP
Plasmid#65300Purposesynchronize trafficking of VSVG from the ER (RUSH system)DepositorInsertVSV-G
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
5xGAL4-TATA-luciferase
Plasmid#46756PurposeLuciferase reporter gene for factors with GAL4 DNA binding domainsDepositorInsertLuciferase
UseLuciferaseTagsExpressionMutationPromoterminimal TATAAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pLenti6.2_mTagBFP2
Plasmid#113725PurposeExpresses the fluorescent protein mTagBFP2 in a lentiviral backboneDepositorInsertmTagBFP2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCB320
Plasmid#89359Purpose3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sitesDepositorInsertpuromycin resistance, mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmNeonGreenHO-mito
Plasmid#127913Purposea mammalian expression vector for mitochondria-targeting humanized mNeonGreenDepositorInserthumanized mNeonGreen with mitocondrial targetting signal
UseTagsmNeonGreenExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_Gal4-VP64-IRES-mCherry (pLZA139)
Plasmid#203909PurposeEncodes wild type Gal4-VP64 transcription factor in mammalian cellsDepositorInsertGal4-VP64-IRES-mCherry
UseTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBA559
Plasmid#186235PurposepTet-LbuCas13a crRNA entry vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXPR_050
Plasmid#96925Purposelentiviral expression of gRNA scaffoldDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_SBP-mCherry-Ecadherin
Plasmid#65289Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorInsertEcadherin
UseTagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only
