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Plasmid#174597PurposeExpresses secreted murine interferon betaDepositorInsertsecreted IFN-beta
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEJS654 All-in-One AAV-sgRNA-hNmeCas9
Plasmid#112139PurposeDelivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.DepositorInsertssgRNA scaffold
Human-codon-optimized NmeCas9
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMammalianMutationPromoterU1a and U6Available sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
FKBP-mCherry
Plasmid#108122PurposeRecruitable fluorophoreDepositorInsertFKBP1A(isoform a, 3-108):mCherry (FKBP1A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ClopHensor
Plasmid#25938DepositorInsertClopHensor
UseTagsStrep-IIExpressionMammalianMutationE2GFP is EGFP-T203YPromoterAvailable sinceSept. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET30a-AlkB-D135S
Plasmid#79051PurposeExpress mutant AlkB protein (D135S) in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
UseTagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E--3mnx1
Plasmid#74632PurposeCan be used to drive expression specifically in zebrafish motor neuronsDepositorInsertmotor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)
Use5' entry vector for multisite gateway three …TagsExpressionMutationPromoter-3mnx1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2B-GFP
Plasmid#116869PurposeNuclear localized GFPDepositorInsertGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-hVAP-A KD/MD
Plasmid#104449PurposeExpression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cellsDepositorInsertvesicle-associated membrane protein-associated protein A (VAPA Human)
UseTagsEGFPExpressionMammalianMutationchanged K94 to D and M96 to DPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-HaloCaMP1b-EGFP
Plasmid#138328Purposechemigenetic calcium indicatorDepositorHas ServiceAAV1 and AAV9InsertHaloCaMP1b
UseAAVTagsEGFP, His-tag, and Nuclear Exclusion SignalExpressionMammalianMutationPromotersynapsinAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGG-B1
Plasmid#78504PurposeTrans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsidDepositorInsertsAAV2 Rep
AAV-B1 capsid
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
p8266 LentiCRISPR v2 hygro sgNT-1
Plasmid#193977PurposeExpression of spCas9 and non-targeting control sgRNADepositorInsertspCas9
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-hPhyB621-mCherry-HRasCT
Plasmid#100282PurposeExpresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cellsDepositorInsertPhytochrome B (1-621 aa) (PHYB Mustard Weed)
UseTagsmCherry fused with HRas C-terminus for plasma mem…ExpressionMammalianMutationHuman codon optimized PhyB (1-621 aa)PromoterCAG promoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1 EYFP H148Q/I152L
Plasmid#25872DepositorInsertEYFP H148Q/I152L
UseTagsExpressionMammalianMutationEYFP from pEYFP (Clontech) with H148Q and I152L m…PromoterAvailable sinceAug. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-iCAM-1-Tether
Plasmid#205187PurposeThis vector encodes of a synCAM (iCAM1 Tether) and GFPDepositorInsertsynCAM iCAM1 tether and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOpen-DpnIR
Plasmid#165529PurposeUsed to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)DepositorInsertDpnI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMXS-IRES-BLAST co hPHGDH
Plasmid#154915PurposeExpress codon optimized human PHGDHDepositorInsertPHGDH, codon optimized (PHGDH Synthetic, Human)
UseRetroviralTagsExpressionMammalianMutationCodon optimized for expression in human cellsPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-T7-Ct-WspR
Plasmid#79166PurposeDual expression of Ct biosensor and WspR WT under individual T7 promotersDepositorInsertsUseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHybrid-Electra2
Plasmid#179475PurposeExpresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cellsDepositorInsertsElectra2
mScarlet-I
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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RP1B FUS 1-163
Plasmid#127192PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 (FUS Human)
UseTagshexahistidineExpressionBacterialMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Non tagged Avidin
Plasmid#214835PurposeThis plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertNon tagged Avidin
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-GFP
Plasmid#86145PurposeLentiviral vector expressing Cas9-GFPDepositorInsertCas9-GFP
UseCRISPR and LentiviralTagsFLAG and GFPExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-DVL1-WT
Plasmid#194586PurposeMammalian Expression of mEGFP-DVL1 WT Fusion ProteinDepositorInsertDVL1 (DVL1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gamillus/ pcDNA3
Plasmid#124837Purposemammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent proteinDepositorInsertGamillus
UseTagsNo tagExpressionMammalianMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-3xFLAG-Rbfox3
Plasmid#213727PurposeMammalian cell expression vector for overexpression of Rbfox3 in motor neurons.DepositorInsertRbfox3 (Rbfox3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF3376
Plasmid#144852PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTOX (TOX Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
mIFP-N1
Plasmid#54620PurposeLocalization: N1 Cloning Vector, Excitation: 683, Emission: 704DepositorTypeEmpty backboneUseTagsmIFPExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRBC-sfGFP-150TAG
Plasmid#174076PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,DepositorInsertSuperfolder GFP
UseTagsHis-6ExpressionBacterialMutationN150TAGPromoterT7Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FLAG-GFP-Tmem192
Plasmid#134630Purposelysosomal markerDepositorInsertTMEM192 (TMEM192 Human)
UseLentiviralTagsFLAG and GFPExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-2xFLAG-SREBP-1c
Plasmid#26802DepositorInsertSREBP-1c (SREBF1 Human)
UseTags2XFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)
Plasmid#197510PurposeCbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)DepositorInsertAAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
UseAAV and CRISPRTagsBPNLS and NpuN(intein)-BPNLSExpressionMutationABE8e mutations in TadA and nSpRY(D10A/A61R)PromoterCbhAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT(CO)-MBIS(CO, ispA)
Plasmid#35151DepositorInsertAtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA
UseSynthetic BiologyTagsExpressionBacterialMutationR364S in Mevalonate KinasePromoterLacUV5,Available sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ
Plasmid#206981Purposeempty pTRIPZ vector for cloning of doxycycline-inducible gene expressionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-mKate2-IRES-MCS (WT-WT)
Plasmid#105921PurposeAAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRsDepositorHas ServiceAAV2InsertsmKate2
IRES-Multiple cloning site
UseAAVTagsExpressionMutationcodon optimizedPromoterCBh and same CBh promoter as mKate2Available sinceFeb. 15, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-HA-hIFITM1
Plasmid#58399PurposeExpressed human IFITM1 with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM1 (IFITM1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-ecfp
Plasmid#65030PurposeGmR mini-Tn7 vector, mobilizable, for CFP tagging bacteriaDepositorInsertecfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
4MT-D3-CPV
Plasmid#58184PurposeLocalization: Mitochondria Ca Biosensor, Excitation: 433 / 515 , Emission: 475 / 528DepositorTypeEmpty backboneUseTagsECFP and VenusExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETDuet-T7-Ct-YhjH
Plasmid#79167PurposeDual expression of Ct biosensor and YhjH under individual T7 promotersDepositorInsertsyhjH
T7-Spinach2-Ct
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only