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Showing: 9921 - 9960 of 156661 results

  1. pDonor_RB-TnV2

    Plasmid
    #213907
    Purpose
    Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites
    Depositor
    James Collins
    Article
    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.
    Insert
    mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213907-plasmid-map-sequence-id-422622

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  11. pOPINB-HOIL-1 helix-RBR (233–510)

    Plasmid
    #193859
    Purpose
    Expression of human His-tagged HOIL-1 (RBCK1) helix-RBR (aa 233–510) codon optimized for E. coli
    Depositor
    Bernhard Lechtenberg
    Article
    Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:
    Insert
    HOIL-1 (RBCK1 Human)
    Use
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    Residues 233–510, codon optimised for expression …
    Promoter
    T7
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    193859-plasmid-map-sequence-id-379616

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  14. KG#901

    Plasmid
    #110936
    Purpose
    Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal
    Depositor
    Kenneth Miller
    Article
    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:
    Inserts
    mig-13 promoter
    Emerald-RAB-3
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110936-plasmid-map-sequence-id-216868

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  17. L3829

    Plasmid
    #1604
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, lacZN
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1604-plasmid-map-sequence-id-214

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  18. Intron-Tagging-EGFP-Frame0-Parental-Minicircle

    Plasmid
    #216124
    Purpose
    Parental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes.
    Depositor
    Stefan Kubicek
    Article
    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d
    Insert
    sgRNA-SA-(GGGGS)x4-EGFP-(GGGGS)x4-SD
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216124-plasmid-map-sequence-id-428729

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  21. HisMS2_VLP_RSV

    Plasmid
    #175958
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]
    Depositor
    Paul Freemont
    Article
    Unruh et al medRxiv 2024.04.17.24305965
    Inserts
    P gene
    M gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 19, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    175958-plasmid-map-sequence-id-357255

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  24. pMXs-IP-mCherry-TOSI

    Plasmid
    #172496
    Purpose
    mCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.
    Depositor
    David Scadden
    Article
    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:
    Insert
    PDCD4 (Pdcd4 Mouse)
    Use
    Retroviral
    Tags
    Mcherry
    Expression
    Mammalian
    Mutation
    Promoter
    MLV LTR
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172496-plasmid-map-sequence-id-338550

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  26. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Use
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182287-plasmid-map-sequence-id-363047

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  33. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4

    Plasmid
    #217895
    Purpose
    Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4)
    Depositor
    Brenda Schulman
    Article
    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    217895-plasmid-map-sequence-id-432156

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  36. pLV-ER GFP

    Plasmid
    #80069
    Purpose
    To express and target GFP into the endoplasmic reticulum using lentivirus
    Depositor
    Pantelis Tsoulfas
    Insert
    ER GFP (SEC61B Human)
    Use
    Lentiviral
    Tags
    GFP derived from Aequorea coerulescens on the NH2…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    80069-plasmid-map-sequence-id-154194

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  39. pRMC2-msfGFP

    Plasmid
    #194913
    Purpose
    Tetracycline inducible expression of msfGFP in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno Sequence followed by monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194913-plasmid-map-sequence-id-384624

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Showing: 9921 - 9960 of 156661 results