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Plasmid#192069PurposeExpression of ADAR2, sesRNA in mammalian cells, with tTA2 as efRNA and ADAR2 overexpression.DepositorInsertsesRNA for mouse Ctip2
UseAAVTagsExpressionMammalianMutationPromoterCbhAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpeCas9-HF
Plasmid#192141PurposeExpresses highly specific SpeCas9, and cloning backbone for sgRNADepositorInsertSpeCas9-HF
UseAAVTagsExpressionMammalianMutationSpeCas9-HF (R247A, N415A, S421A)PromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pyc111f
Plasmid#158780Purposeribosome-tagged, Cre-dependent GCaMP for soma localizationDepositorInsertGCaMP6f-RPL10a
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pyc111m
Plasmid#158779Purposeribosome-tagged, Cre-dependent GCaMP for soma localizationDepositorInsertGCaMP6m-RPL10a
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
scADGFP
Plasmid#114434PurposeEpilepsy promoter enhanced with Arc elements driving GFP with SV40E polyADepositorInsertGreen fluorescent protein
UseAAVTagsER export sequence FCYENEVExpressionMammalianMutationPromoterNovel synthetic activity-dependent promoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6sgbbBsmbI-puro-2A-Fluc
Plasmid#100277PurposeLentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imagingDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
CSAC-Pers
Plasmid#168281PurposeExpresses sgRNA in mammalian cellsDepositorInserthU6-sgPer1-3-hU6-sgPer2-1-hU6-sgPer2-2
UseAAVTagsmCherryExpressionMutationPromoterU6, hSynAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
CSAC-Crys
Plasmid#164967PurposeExpresses sgRNA in mammalian cellsDepositorInserthU6-sgCry1_2-hU6-sgCry2_1-hU6-sgCry2_2
UseAAVTagsmCherryExpressionMutationPromoterhU6, hSynAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_DIO_R106W-HaloTag
Plasmid#164063PurposeDouble-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R106W mutationDepositorInsertMeCP2 R106W (Mecp2 Mouse)
UseAAVTagsHaloTagExpressionMammalianMutationPromoterEF1AAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pyc128m
Plasmid#158778Purposesoluble GCaMP expressionDepositorInsertGCaMP6m
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sha2Cas9-HF
Plasmid#192142PurposeExpresses highly specific Sha2Cas9, and cloning backbone for sgRNADepositorInsertSha2Cas9-HF
UseAAVTagsExpressionMammalianMutationSha2Cas9-HF (R247A and N415A)PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCsy3-VPR-Csy4
Plasmid#153943PurposeExpresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy3-VPR and Csy4 in mammalian cellsDepositorInsertCsy3-VPR, Csy4
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCsy1-Csy2
Plasmid#153942PurposeExpresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy1 and Csy2 in mammalian cellsDepositorInsertCsy1, Csy2
UseAAV and CRISPRTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
IL1RN gRNA3
Plasmid#113132PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
CamKII-mtPyronicSF
Plasmid#228906PurposeExpression of mtPyronicSF in neurons targeted to the mitochondria serving as an optical sensor for mitochondrial pyruvate uptake. Backbone from Addgene plasmid 100834DepositorInsertpyronic SF
UseAAVTagsCox8 targettingExpressionMammalianMutationPromoterCamKIIAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX552-hsyn DIO GCaMP8f(with gRNA scaffold)
Plasmid#199580PurposeExpresses GCaMP8f in a Cre-dependent mannerDepositorInsertHis-DIO GCaMP8f
UseAAV, CRISPR, and Cre/LoxTags6xHisExpressionMammalianMutationPromoterhsynAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552-EF1a-DIO Chronos-eGFP(with gRNA scaffold)
Plasmid#199582PurposeExpresses Chronos-eGFP in a Cre-dependent mannerDepositorInsertChronos eGFP
UseAAV, CRISPR, and Cre/LoxTagsGFPExpressionMammalianMutationPromoterEF1a intron AAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNF130 shRNA
Plasmid#225340PurposeshRNAmir backbone for RNA interference, with YFPDepositorInsertRNF130 shRNA (Rnf130 Mouse)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterCBAAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
USP7 shRNA-TRE
Plasmid#225338PurposeshRNAmir backbone under tet-operator for RNA interference, with YFPDepositorInsertUSP7 shRNA (Usp7 Mouse)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterTRE (tetracycline-responsive element)Available sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1α:flex-mcherry
Plasmid#222618PurposeCre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoterDepositorInsertCre-dependent mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only