Showing: 4941 - 4960 of 16015 results
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Plasmid#109031PurposeD16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
UseTagsFlag TagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag H588A
Plasmid#109032PurposeH588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
UseTagsFlag TagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
RNA polymerase beta prime subunit FLAG
Plasmid#102337PurposeTargeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.DepositorInsertRNAP Beta Prime Subunit with C-terminal FLAG TAG
UseTags3x FLAG with 3X GSGS linkerExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTRE-tight2-Tmem98-FLAG
Plasmid#124506PurposeExpresses FLAG-tagged mouse TMEM98 in mammalian cells in a doxycycline-dependent manner (C-terminal tag)DepositorInsertTmem98 (Tmem98 Mouse)
UseTagsFlagExpressionMammalianMutationPromotertetO-CMVAvailabilityAcademic Institutions and Nonprofits only -
N8his-GFPenhancer-GGGGS4-LaG16
Plasmid#140442PurposeWe designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.DepositorInsertGFPenhancer-GGGGS4-LaG16
UseTags8 histidine tagExpressionBacterialMutationPromoterT7 promotorAvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP2
Plasmid#135551PurposeExpress mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP2
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP3
Plasmid#135552PurposeExpress rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP3
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP8
Plasmid#135553PurposeExpress mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP8
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAPromoterAvailabilityAcademic Institutions and Nonprofits only -
mCherry-NbALFA
Plasmid#159987PurposeFor the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmCherry-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mCherry and NbALFAPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28a-TS2126 RnlA-Strep
Plasmid#159350PurposeExpress TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectivelyDepositorInsertTS2126 RNA ligase
UseTagsHis6 and StrepExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
C514-E12: attB5r-RU5u-His6-eGFP-nostop-attB1r
Plasmid#162954PurposeGateway attB5r/attB1r entry clone for generation of N-terminal eGFP-tagged fusion proteinsDepositorInsertHis6-Green fluorescent protein fusion tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
C514-E13: attB5r-RU5u-His6-GST-nostop-attB1r
Plasmid#162955PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertHis6-Glutathione-S-transferase fusion tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
C514-E15: attB5r-RU5u-His6-MBP-nostop-attB1r
Plasmid#162957PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertHis6-Maltose-binding protein fusion tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r
Plasmid#162964PurposeGateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expressionDepositorInsertHis6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-W741C-S81A-P82A
Plasmid#171250PurposeMammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Ala (AR-W741C-S81A-P82A)DepositorInsert3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Ala mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Trp741Cys-Ser81Ala-Pro82Ala (AR-W741C-S81A-P82…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-W741C-S81A-P82G
Plasmid#171251PurposeMammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82G)DepositorInsert3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Gly mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pTU398
Plasmid#178980Purpose13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)DepositorInsertgRAMP
UseTagsStrep-Tag II SUMOExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EKAR4
Plasmid#174437PurposeImproved cyan/yellow FRET-based ERK kinase activity reporter.DepositorInsertEKAR4
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-cytoEKAR4
Plasmid#174438PurposeCytosol-targeted EKAR4.DepositorInsertcytoEKAR4
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 4941 - 4960 of 16015 results