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Plasmid#153497PurposeAspergillus awamori Glucoamylase Signal Peptide Donor PlasmidDepositorInsertGlucoamylase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Gga-Lysozym SP
Plasmid#153505PurposeChicken Lysozym Signal Peptide Donor PlasmidDepositorInsertLysozym SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_TEV-His-GFP11
Plasmid#153510PurposeTEV-His-GFP11 C-terminal Tag Donor PlasmidDepositorInsertTEV-His-GFP11
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Sce-Prepro SP
Plasmid#153491PurposeS. cerevisiae Prepro Signal Peptide Donor PlasmidDepositorInsertPrepro SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
A102-GAP-BoCCS
Plasmid#123287PurposeExpression of truncated Glo3 (1-375) in E.ColiDepositorInsertGlo3 (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutation1-375PromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-BoCCS-GRM
Plasmid#123288PurposeExpression of truncated Glo3 (137-459) in E.ColiDepositorInsertGlo3 (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutation137-459PromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-Par-BoCCS-GRM
Plasmid#123289PurposeExpression of truncated Glo3 (296-459) in E.ColiDepositorInsertGlo3 (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutation296-459PromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-BoCCS
Plasmid#123290PurposeExpression of truncated Glo3 (137-375) in E.ColiDepositorInsertGlo3 (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutation137-375PromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pEDJ-22
Plasmid#140904PurposeMarkerFree plasmid for integration of pADH1-PCP-Mxi1 into site X-4DepositorInsertPCP-Mxi1
UseTagsExpressionMutationPromoterADH1Available sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS424-ysg(NT)
Plasmid#138258PurposeExpression of a non-targeting sgRNA to be used as a control in a yeast dual reporter systemDepositorInsertNontargeting sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-ysg(NT)
Plasmid#138263PurposeSubcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.DepositorInsertNontargeting sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAL- S. pombe TSC1 (1-431)
Plasmid#132616Purposeexpresses S. pombe TSC1 (1-431) in E. coliDepositorInsertTSC1 (1-431)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMY07AB-PRO
Plasmid#127710PurposeLevel 0 destination vector for a promoter with A - B scars. Clone in with BpiIDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Spt2-K166R-GFP
Plasmid#115573PurposeExpresses yeast Spt2-GFP fusion protein mutated from lysine to arginine at site 166DepositorInsertSPT2
UseTagsGFPExpressionBacterial and YeastMutationLysine 166 mutated to ArgininePromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28 SpHIS5 (pNTI663)
Plasmid#115433PurposeExpresses wild-type RPL28 from HIS3 yeast plasmidDepositorInsertsUseTagsExpressionYeastMutationMfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(Q38P) SpHIS5 (pNTI664)
Plasmid#115434PurposeExpresses Q38P RPL28 from HIS3 yeast plasmidDepositorInsertsUseTagsExpressionYeastMutationQ38P; MfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(H39L) SpHIS5 (pNTI665)
Plasmid#115435PurposeExpresses H39L RPL28 from HIS3 yeast plasmidDepositorInsertsUseTagsExpressionYeastMutationH39L; MfeI site in intronPromoterashbya gospii TEF1 and endogenousAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS315Nab3D191stop
Plasmid#110537PurposeExpresses yeast NAB3 using the endogenous promoterDepositorInsertNAB3 (NAB3 Budding Yeast)
UseTagsExpressionYeastMutationC-terminal deltion of 191 amino acidsPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
PP245
Plasmid#78965PurposePromoter: GAP, minimal Promoter: AOX1. Figure 4. Expresses GFP.DepositorInsertGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only