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pEX-PK-hLC3

Plasmid

#61458

Purpose

Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy

Depositor

Isei Tanida

Article

Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

Insert

PK-hLC3 (MAP1LC3B Human, Fission Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pEX-PK-hLC3∆G

Plasmid

#61459

Purpose

Express pHluorin-mKate2-hLC3∆G (PK-hLC3∆G), a negative control for pHluorin-mKate2-hLC3 (PK-hLC3), in mammalian cells for monitoring autophagy

Depositor

Isei Tanida

Article

Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

Insert

PK-hLC3∆G (MAP1LC3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-ACTB

Plasmid

#207748

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of ACTB (ACTB Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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RpH-ILV-3xALFA

Plasmid

#225135

Purpose

RpH-ILV probe strongly colocalises with lysosomal markers.

Depositor

Massimiliano Stagi

Article

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

TMEM192 (TMEM192 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-COX8A

Plasmid

#227308

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of COX8A for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of COX8A (COX8A Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U67

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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iCAP-BI-28-KanR

Plasmid

#203532

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

AAV-BI28 Cap

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-inactive M3nls

Plasmid

#157854

Purpose

Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)

Use

Tags

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A; METTL3 D395A

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Polymerase expression - pCMV-T7-EcKlenow (LM2692)

Plasmid

#208963

Purpose

Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

EcKlenow-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Px461-Cas9n-Trp53-sgRNA-beta

Plasmid

#88847

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53 (Trp53 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
Px461-Cas9n-Trp53-sgRNA-alpha

Plasmid

#88846

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53 (Trp53 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)

Plasmid

#149276

Purpose

Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence

Use

T-dna vector

Tags

Expression

Mutation

T2318C (DNA) in PEBV coat protein sequence

Promoter

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027

Plasmid

#134611

Purpose

Mammalian expression of mouse KIF5A 378-1027

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5A (Kif5a Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 377 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-COSMC-KO

Plasmid

#80008

Purpose

gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

Depositor

Sriram Neelamegham

Article

Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

Insert

COSMC (C1GALT1C1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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T7_CC_PE7_IVT_Template

Plasmid

#223022

Purpose

Template for in vitro transcription of PE7. For HSPC-related experiments.

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mutation

Promoter

T7

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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CGBE1-NG (pBM1246)

Plasmid

#140256

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)_NG-ΔUGI

Use

Tags

Expression

Mammalian

Mutation

R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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RpH-ILV-FRB

Plasmid

#225137

Purpose

RpH-ILV probe strongly colocalises with lysosomal markers with FRB

Depositor

Massimiliano Stagi

Article

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

TMEM192 (TMEM192 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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BLBP-GFP

Plasmid

#63174

Purpose

Expressing GFP from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

Depositor

Bart De Strooper

Article

Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

Insert

BLBP (Fabp7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

1700bp upstream of BLBP gene

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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CRISPR-SB

Plasmid

#177936

Purpose

Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

Depositor

Roland Rad

Article

Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

Type

Empty backbone

Use

CRISPR; Transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL11060

Plasmid

#68264

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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H3K9me3 biosensor

Plasmid

#120802

Purpose

FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Truncated YPet-HP1-EV linker-ECFP-mouse histone H3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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