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pMRE-Tn7-144

Plasmid

#118560

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mOrange2

Use

Minitn7 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-154

Plasmid

#118536

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mOrange2

Use

Minitn5 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-LKB1-KM

Plasmid

#21148

Depositor

Junying Yuan

Article

Karuman et al Mol Cell. 2001 Jun . 7(6):1307-19.

Insert

GFP-LKB1-KM (Stk11 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

K78M

Promoter

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only
AAV-shRNA-Tet2

Plasmid

#85743

Purpose

shRNA against mouse Tet2

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

shRNA Tet2

Use

AAV

Tags

EYFP

Expression

Mutation

Promoter

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pSF-p15A-YFP

Plasmid

#186563

Purpose

p15A ori, promotorless, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Use

Tags

None

Expression

Bacterial and Mammalian

Mutation

None

Promoter

Promotorless

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 T18N

Plasmid

#47897

Purpose

Expresses myc tagged Miro2 T18N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 T18N (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

T18N, dominant negative mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 A13V

Plasmid

#47896

Purpose

Expresses myc tagged Miro2 A13V constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 A13V (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

A13V, constitutively active mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pCS2-PLK1-mCherry

Plasmid

#127154

Purpose

Expresses mCherry PLK1 in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2019 Jun 15;30(13):1598-1

Insert

PLK1-mCherry (PLK1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-RaichuEV-Rac1(T17N)

Plasmid

#164902

Purpose

Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

RaichuEV-Rac1 (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

T17N mutation in the Rac1 domain which make the s…

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-VP160-pU6-sgRNA

Plasmid

#158987

Purpose

Vector D encodes pAAV-pMecp2-dSaCas9-VP160-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP160

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pMecp2

Available since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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GST-TPX2(1-43)

Plasmid

#69107

Purpose

For bacterial expression of human TPX2(1-43aa) tagged with GST.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Targeting Protein for Xklp2 (TPX2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Amino acids 1-43 expressed.

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pHB9-MCS-IRES-EGFP (mHb9 promoter) [TJ#103]

Plasmid

#16283

Depositor

Thomas Jessell

Article

Wilson et al J Neurosci. 2005 Jun 15. 25(24):5710-9.

Insert

HB9 promoter (Mnx1 Mouse)

Use

Mouse Targeting

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-mCherry-TSC2-NLS

Plasmid

#192441

Purpose

Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2-NLS (TSC2 Human)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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MSP1594

Plasmid

#65775

Purpose

Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-165

Plasmid

#118577

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet

Use

Minitn7 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1a2

Plasmid

#13751

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1a2 (MAPK8 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
trPAGmEos3.2

Plasmid

#203752

Purpose

Encodes the transmembrane domain from PAG/CSK, including its 2 palmitoylation sites, with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trPAG (PAG1 Human)

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pUB_smFLAG_H2B_MS2

Plasmid

#81082

Purpose

Translation reporter: Encodes Histone H2B (125 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smFLAG_H2B_24xMS2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax

Plasmid

#173874

Purpose

Mammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pL-SFFV.Reporter.RFP657.PAC

Plasmid

#61395

Purpose

Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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