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Plasmid#63154PurposeMiddle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLSDepositorInsertCas9
UseCRISPR; Tol2 middle entry vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_TOP-iRFP_L5-L4
Plasmid#113866PurposeSingle pathway reporter; wnt promoter-driven expression of iRFP; MultiSite Gateway entry vector.DepositorInsertsTOP (wnt dependent promoter)
iRFP713
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-U6:gRNA
Plasmid#63156PurposeDestination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3
Plasmid#113864PurposeSingle pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.DepositorInsertsPTCH1 (hedgehog dependent promoter)
mTurquoise2
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSC6
Plasmid#104780PurposepSC218UG-At Ubiquitin10:Gateway expression cassette binary vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-ERN
Plasmid#80475PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and neomycin resistance) aka. KW111DepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-TA-ERN
Plasmid#80474PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-Lifeact
Plasmid#109545PurposeMultisite gateway vector for 5' tagging with the actin binding motif LifeactDepositorInsertactin binding motif Lifeact
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pDONR221_SLC22A1
Plasmid#132034PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC22A1 (SLC22A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-FLAG
Plasmid#183511PurposeGateway converted, destination vector with N-terminal Flag tag. For mammalian expression.DepositorTypeEmpty backboneUseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5E-mfap4
Plasmid#70052Purpose5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.DepositorInsertmfap4 promoter
UseGateway 5' elementTagsExpressionMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pAAV2-DEST(f)
Plasmid#190226PurposeGateway destination vector, empty AAV vector backboneDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-mScarlet
Plasmid#140875PurposeMultisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.DepositorInsertmScarlet
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
YFC43
Plasmid#89488Purposegateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing C terminal fragment of YFPDepositorTypeEmpty backboneUseTagsYFP-C (aa 154-240)ExpressionPlantMutationPromoter2x35SAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-TET
Plasmid#20909PurposepiggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)DepositorInsertGateway Destination Vector
UsepiggybacTagsIRES-bGeoExpressionMammalianMutationPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
YFN43
Plasmid#89487Purposegateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing N terminal fragment of YFPDepositorTypeEmpty backboneUseTagsYFP-N (aa 1-154)ExpressionPlantMutationPromoter2x35SAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3C WT
Plasmid#123095PurposeExpresses 3xFLAG-LC3C wild-type in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDestattB_ubi:EGFP (pCM272)
Plasmid#68339PurposeMultisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.DepositorInsertEGFP
UseZebrafish transgenesisTagsExpressionMutationPromoterubiAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only