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Plasmid#213744PurposeFor circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cellsDepositorInsertLbCas12a
UseCRISPRTagsBPNLSExpressionMammalianMutationD156RPromoterCMVAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Lifeact-7
Plasmid#54491PurposeLocalization: Action, Excitation: 587, Emission: 610DepositorTypeEmpty backboneUseTagsmCherry-LifeactExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
Dox nontargeting shRNA mKate
Plasmid#155292PurposeLentivirus for doxycycline inducible expression of nontargeting shRNA, mKate marker, based on Addgene 11652DepositorInsertNontargeting shRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)
Plasmid#185912PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpRY-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VSVL
Plasmid#64085PurposeExpression vector for Vesicular Stomatitis Virus Indiana strain polymerase geneDepositorInsertVSV polymerase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-EF1a-i53
Plasmid#216243PurposeExpress i53 in mammalian cells as a HDR enhancerDepositorInserti53
UseTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGCC4
Plasmid#37058DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJune 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-2C-NLS in pCSDest
Plasmid#126637PurposeExpresses AsCas12a-2C-NLS in mammalian cellsDepositorInsertAsCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-mCherry
Plasmid#50459PurposeDouble floxed mCherry under the control of human synapsin promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertmCherry
UseAAVTagsN/AExpressionMutationPromoterhuman Synapsin 1Available sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-WHaloCaMP1a
Plasmid#205310PurposeProduction of AAV particles encoding WHaloCaMP1a from the CaMKII promoterDepositorInsertWHaloCaMP1a
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX NPC1-FLAG-TiD
Plasmid#199748PurposeLentiviral construct to stably express NPC1 TurboIDDepositorInsertNPC1 (NPC1 Human)
UseTagsFlag and TurboIDExpressionMammalianMutationPromoterEF1aAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0500
Plasmid#143709PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPPARGC1A (PPARGC1A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-OVOL2-HA
Plasmid#184737PurposeDoxycycline-inducible lentiviral expression of OVOL2DepositorInsertovo like zinc finger 2 (OVOL2 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterTet ONAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mm-PylRS-AF/Pyl-tRNACUA
Plasmid#122650PurposetRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.DepositorInsertPylRS-AF, Pyl-tRNACUA
UseTagsFLAGExpressionMammalianMutationTyrosine 306 changed to Alanine, Tyrosine 384 cha…PromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2EmGFP-DAXX
Plasmid#169726PurposeMammalian expression construct of GFP tagged full length DAXXDepositorInsertDAXX (DAXX Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET16b-PCNA
Plasmid#134898PurposeBacterial expression and purification of human PCNADepositorInsertPCNA (PCNA Human)
UseTagsuntaggedExpressionBacterialMutationfully synthetic, codon optimized for bacterial ex…PromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEN276
Plasmid#69150PurposeIntegration of luciferase operon in Tn7 insertion site of Gram negative bacteriaDepositorInsertluxCDABE
UseLuciferaseTagsExpressionMutationPromoterfrrAvailable sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-TO-ZscHCVP-TEVPZ
Plasmid#116094PurposeExpresses a combination of proteasesDepositorInsertZscHCVP-TEVPZ
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2NS-IRES-dOrai-IRES-GFP
Plasmid#72895PurposeMammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFPDepositorInsertdmBACCS2NS-IRES-dOrai-IRES-GFP
UseTagsIRES-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Flag-hsDicer
Plasmid#41584DepositorInserthsDicer (DICER1 Human)
UseTagsFlagExpressionMammalianMutationNonePromoterCMVAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only