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Plasmid#145362PurposeCell-free/mammalian expression vector for Zika virus NS1 protein with N-term mCherryDepositorInsertZIKV NS1
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pmCell-Free_KA2_ZIKV NS2B
Plasmid#145364PurposeCell-free/mammalian expression vector for Zika virus NS2B protein with N-term mCherryDepositorInsertZIKV NS2B
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2_ZIKV NS3
Plasmid#145365PurposeCell-free/mammalian expression vector for Zika virus NS3 protein with N-term mCherryDepositorInsertZIKV NS3
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pTSK450
Plasmid#34552DepositorInsertMS2-CP-NLS
UseTagsmCherryExpressionYeastMutationPromoterMet25Available sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.chFP.W
Plasmid#51008PurposeLentivector that expresses chFP from internal human PGK promoterDepositorInsertmChFP
UseLentiviralTagsExpressionMammalianMutationPromoterhuman PGKAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SIM)3
Plasmid#127148PurposeMammalian expression plasmid for mCherry-(SIM)3 client to assess SUMO-SIM scaffolds.DepositorInsert3 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-mCh
Plasmid#80492Purposedonor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expressionDepositorInsertmCherry
UseDonor vector (human)TagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
LLP618_αGCN4-KRAB-CO
Plasmid#211782PurposeSunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selectionDepositorInsertaGCN4-mCherry
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect mCherr…PromoterpEF1a and pSV40Available sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS132
Plasmid#211503PurposeAAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seqDepositorInsertmCherry
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-Scramble
Plasmid#175014PurposeNegative-control construct for Akt-STOPS.DepositorInsertmCherry-Akt-Scramble
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-STOPS-NLS
Plasmid#175007PurposeNuclear-targeted Akt-STOPS.DepositorInsertmCherry-Akt-STOPS-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPEPZ-sgRNAclone
Plasmid#141090PurposeThis vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.DepositorInsertmCherry
UseCRISPRTagsExpressionBacterialMutationPromoterp3Available sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
LENC
Plasmid#111163PurposemiR-E (miR-30 variant)-based RNAi with mCherry reporterDepositorInsertmiR-E (miR-30 variant)
UseRetroviralTagsExpressionMutationWTPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PdTK-CAG-mCh-[uBglII]
Plasmid#107280Purposepuro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherryDepositorInsertAAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )
UseTagsExpressionMutationPromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRubiC-T2A-Cas9
Plasmid#75347PurposeUbiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.DepositorInsertCas9
UseCRISPR and RetroviralTagsmCherry (via t2a)ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSNV2 CHER NLS cop1at(335-675)
Plasmid#44972DepositorInsertmCherry-NLS-COP1 (COP1 Mustard Weed)
UseTagsNLS and mCherryExpressionMammalianMutationdeleted amino acids 1-334PromoterpCMV IEAvailable sinceJune 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-Akt-STOPS
Plasmid#175009PurposePlasma membrane-targeted Akt-STOPS.DepositorInsertLyn-mCherry-Akt-STOPS
UseTagsN-terminal targeting sequence from Lyn kinase and…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pASPIre1
Plasmid#154842PurposeDerivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sitesDepositorInsertBxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
UseTagsExpressionBacterialMutationPromoterPrhaAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAN2981
Plasmid#127246PurposeAsymmetric degronSwitchA_t8 fused to mCherry with KeyA-BFPDepositorInsertpPGK-Key_A_e18-TagBFP-P2A-mCherry-asym_degronSwitch_A_t8-WPRE
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDM1383
Plasmid#207423PurposeExtrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)DepositorInsertsUseUnspecifiedTagsGFP and mCherryExpressionMutationaa 1–134 (RBD) onlyPromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only