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Plasmid#208254PurposepGL3 promoter luciferase reporter vector containing 3 copies of the NBRE DNA response element (5′-GAGTTTTAAAAGGTCATGCTCAATT TGTC-3′) used for luciferase reporter assays in mammalian cellsDepositorInsert3X NBRE-LUC
UseTagsExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1329 - pAAV EF1a HA-hM4D(Gi)
Plasmid#89150PurposeAn AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.DepositorInsertHA-tagged hM4D(Gi)
UseAAVTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
MICU-APEX2
Plasmid#79057PurposeA C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1DepositorInsertC-terminal APEX2 fused MICU1 (MICU1 Soybean, Human)
UseLentiviralTagsFLAGExpressionBacterial and MammalianMutationPromoterCMV immearly promotorAvailable sinceSept. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1328 - pAAV EF1a HA-hM3D(Gq)
Plasmid#89149PurposeAn AAV packaging plasmid that expresses HA-tagged hM3D(Gq) excitatory DREADD from the EF1a promoter.DepositorInsertHA-tagged hM3D(Gq)
UseAAVTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRmine-eYFP-WPRE
Plasmid#130988PurposeAAV vector to drive the expression of ChRmine-eYFP under the control of CamKIIa promoterDepositorInsertChRmine
UseAAVTagseYFPExpressionMutationPromoterCaMKIIaAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-tdTomato-WPRE-pGHpA
Plasmid#67527Purpose"Expresses the fluorescent protein tdTomato"DepositorInserttandem dimer tomato
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pECE HA AMPKa1 WT
Plasmid#69504PurposeExpresses AMPK alpha1 in mammalian cellsDepositorInsert5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO ChRmine-eYFP-WPRE
Plasmid#130996PurposeDouble floxed ChRmine-eYFP under the control of Ef1a promoterDepositorInsertChRmine
UseAAVTagseYFPExpressionMutationPromoterEf1aAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
cyt-GafD-mTurboID-V5
Plasmid#184640PurposeExpresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome taggingDepositorInsertcyt-GlycoID-V5
UseTagsNuclear export sequence and V5 tagExpressionMammalianMutationGafD -> expressed aa22-178 ; BirA* -> expre…PromoterCMVAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVE-hTGFB3-IRES-Red
Plasmid#52580Purpose2nd generation lentiviral transfer vector driving expression of human TGF-beta3 and RFP in mammalian cellsDepositorInserthuman transforming growth factor-beta3 (TGFB3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1alphaAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BLRR
Plasmid#158958PurposeBioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).DepositorInsertBLRR
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOS003-lenti-CMV-GCaMPer
Plasmid#65227PurposeER calcium sensor for lentivirus productionDepositorInsertGCaMPer
UseLentiviralTagscircularly permuted GFPExpressionMammalianMutationGCaMP3 (D324G, D360G, D397G, D435G)PromoterCMVAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1
Plasmid#194279PurposeExpresses gRNA and VP64-dSaCas9-VP64 from lentiviral vectorDepositorInsertshumanized VP64 dSaCas9 VP64 T2A Thy1.1
gRNA
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhU6 and hUbCAvailable sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B EDD
Plasmid#37188DepositorInsertEDD (UBR5 Human)
UseTags6x His Tag and FLAGExpressionMammalianMutationPlease see Depositor CommentsPromoterCMVAvailable sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-fDIO-somQuasAr6a-EGFP-P2A-sombC1C2TG
Plasmid#183537PurposeVoltage indicatorDepositorInsertsomQuasAr6a-EGFP-P2A-sombC1C2TG
UseAAVTagsExpressionMutationNonePromoterhSynAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9-pAc
Plasmid#162163PurposeExpresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectableDepositorInsertshSpCas9
sgRNA
UseCRISPRTagsT2A-pAcExpressionInsectMutationPromoterAe. aegypti PUb and Ae. aegypti U6Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mCherry-IRES-Dre
Plasmid#55633PurposeExpresses Dre in Mammalian CellsDepositorInsertsmCherry
Dre
UseAAVTagsExpressionMammalianMutationPromoterEf1a and Ef1a/IRESAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-CVS-G-WPRE-pGHpA
Plasmid#67528Purpose"Expresses the rabies glycoprotein"DepositorInsertRabies virus strain CVS-11 glycoprotein (G)
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-U6-gRNA-UbC-DsRed-P2A-Bsr
Plasmid#83919PurposeLentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastRDepositorInsertsDsRed-Express2
Bsr
UseCRISPR and LentiviralTagsP2AExpressionMutationPromoterhUbCAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-GCaMP6s-WPRE-pGHpA
Plasmid#67526Purpose"Fluorescent reporter for calcium imaging"DepositorInsertGCaMP6s
UseAAVTags6xHis, T7 tag, and Xpress epitopeExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only