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Plasmid#199573PurposeExpresses FLAG-tagged WT SMG1DepositorInsertSMG1 (SMG1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-JMJD2B
Plasmid#24181PurposeMammalian expression of human JMJD2B with HA tagDepositorInsertJMJD2B (KDM4B Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSOUP
Plasmid#165419PurposeThe pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in AgrobacteriumInsertRep A
UseHelper plasmid required for replication for all p…TagsExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSS9
Plasmid#71655PurposeContains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coliDepositorInsertgfpuv
UseSynthetic BiologyTagsExpressionMutationPromoterT7A1Available sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP
Plasmid#221233PurposeExpress sgRNA targeting HEK3 loci with nCas9(D10A)DepositorInsertSpCas9(D10A)-P2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-TFE3
Plasmid#38120DepositorInsertTFE3 (TFE3 Human)
UseTagsEGFPExpressionMammalianMutationdeleted stop codonPromoterCMVAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLX303-ZIM3-KRAB-dCas9
Plasmid#154472PurposeExpresses ZIM3 KRAB domain fused to the N terminus of dCas9DepositorInsertZIM3 (ZIM3 Synthetic)
UseLentiviralTagsHAExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLT3-ITD-MSCV-IRES-GFP
Plasmid#184779PurposeExpresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cellsDepositorInsertFLT3 (FLT3 Human)
UseRetroviralTagsExpressionMammalianMutationActivating internal tandem duplication (ITD) adde…PromoterMSCVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_25bpLinker
Plasmid#157788PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_25bp linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-eGFP
Plasmid#32548DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterConsitutive lacAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-KO
Plasmid#17603PurposeMammalian expression of HA tagged ubiquitin carrying zero lysines (K0)DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationKO: all lysines mutated to argininesPromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_15bpLinker
Plasmid#157786PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_15bp linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_20bpLinker
Plasmid#157787PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_20bp linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-puro
Plasmid#125839Purposemodifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
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mAzurite-16xRhoBAST
Plasmid#196347PurposeExpression of mAzurite mRNA with 16xRhoBAST in 3'UTRDepositorInsertmAzurite-16xRhoBAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_35bpLinker
Plasmid#157790PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_35bp linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
DT12A
Plasmid#80416PurposeExpresses 12 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX304-Flag-APEX2-NES
Plasmid#92158PurposeCytosolic APEX2 constructDepositorInsertFlag-APEX2-NES
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCasRx-RBM25
Plasmid#221001PurposeTransient expression of dCasRx-RBM25. EF1a promoter.DepositorInsertdCasRx-RBM25
UseCRISPRTagsNLS and NLS-HAExpressionMammalianMutationPromoterEF1aAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only