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pBactin-mNeonGreen-CI

Plasmid

#196850

Purpose

Expression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mNeonGreen

Use

Tags

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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Thy-Kv2.1-HA

Plasmid

#111541

Purpose

For expression of human KCNB1 in mouse brain

Depositor

Federico Sesti

Article

Yu et al J Neurosci. 2016 Oct 26;36(43):11084-110

Insert

thy1.2-Kv2.1-HA (KCNB1 Human)

Use

Mouse Targeting

Tags

HA

Expression

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEF Doc2beta 6X mutant GFP

Plasmid

#128823

Purpose

To visualize Doc2beta 6X mutant in PC12 cells

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D163A, D218A, D220N, D303A, D357A, D359A

Promoter

EF-1alpha

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Gp88 in situ probe

Plasmid

#45628

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Gpr88 in situ probe (Gpr88 Mouse)

Use

In situ

Tags

Expression

Mutation

fragment contains bp# 2742-3255 of AB042408.1 or …

Promoter

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Epha3 in situ probe

Plasmid

#45627

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Epha3 in situ probe (Epha3 Mouse)

Use

In situ

Tags

Expression

Mutation

fragment contains bp# 3179-3773 of NM_010140

Promoter

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-lox-roxSTOProx-lyn-mScarlet-pA-lox

Plasmid

#196889

Purpose

Dre-activated Cre-inactivated expression of lyn-mScarlet reporter.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

lyn-mScarlet

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pX552 EF1a DIO Chronos GFP_Vgat gRNA

Plasmid

#215277

Purpose

Expresses Vgat gRNA in a Cre dependent Chronos GFP vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Vgat gRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pX552 EF1a DIO Chronos GFP_VgatTTT gRNA

Plasmid

#215278

Purpose

Expresses Vgat Control gRNA in a Cre dependent Chronos GFP vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Vgat control gRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pX552-hSyn-DIO-GCaMP8f_Grin1 gRNA

Plasmid

#215281

Purpose

Expresses Grin 1 gRNA in a Cre dependent GCaMP8f vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Grin1 gRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

See Depositor Comments Below

Promoter

U6

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Plasmid

#217509

Purpose

KD Recombinase dependent conditional 1X ALFA cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Use

Tags

ALFA tag

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-deltaExon17

Plasmid

#209563

Purpose

Mammalian expression of Shtn1 long isoform with E17 deletion

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with E17 deletion (4930506M07Rik Mouse)

Use

Tags

Expression

Mammalian

Mutation

Exon17 deletion

Promoter

Available since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-SHTN1LRRR>GGG

Plasmid

#209562

Purpose

Mammalian expression of Shtn1 long isoform with RG mutation

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with RG mutation (4930506M07Rik Mouse)

Use

Tags

Expression

Mammalian

Mutation

RRR to GGG

Promoter

Available since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-deltaExon16-17

Plasmid

#209564

Purpose

Mammalian expression of Shtn1 long isoform with E16-17 deletion

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with E16-17 deletion (4930506M07Rik Mouse)

Use

Tags

Expression

Mammalian

Mutation

Exon16 and 17 deletion

Promoter

Available since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-deltaExon15-II

Plasmid

#209566

Purpose

Mammalian expression of Shtn1 long isoform with E15 deletion II

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with E15 deletion II (4930506M07Rik Mouse)

Use

Tags

Expression

Mammalian

Mutation

Exon15 deletion II

Promoter

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-bIV spectrin (repeats 13-15)

Plasmid

#213728

Purpose

Mammalian cell expression vector for overexpression of bIV spectrin (repeats 13-15) in NIH/3T3 cells.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

bIV spectrin (repeats 13-15) (Sptbn4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGIG-FLAG-SHTN1L

Plasmid

#209568

Purpose

Mammalian expression of Shtn1 long isoform

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform (4930506M07Rik Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGIG-FLAG-SHTN1S

Plasmid

#209569

Purpose

Mammalian expression of Shtn1 short isoform

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 short isoform (4930506M07Rik Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-Halo-Tag

Plasmid

#196898

Purpose

Expression of Halo-Tag. Use as control for Halo-tag fusions

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Halo-Tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcS-RDG-C1C2

Plasmid

#200163

Purpose

This plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.

Depositor

Masako Harada

Article

Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)

Inserts

RDG-C1C2
C1C2 domain of lactadherin

Use

Tags

HA and HIS

Expression

Mammalian

Mutation

sequence adopted from doi: 10.1371/journal.pone.0…

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-AcGFP-CI

Plasmid

#196849

Purpose

Expression of green fluorescent reporter AcGFP driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

AcGFP

Use

Tags

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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