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Plasmid#101373PurposeName: pBluescript-RBPjkΔB. RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).DepositorInsertRBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).
UseUnspecifiedTagsExpressionMutationRBPjk binding site B mutation in pBluescript-LANA…PromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
p35S::AtSOBIR1-YFPc
Plasmid#86162PurposeSOBIR1 split YFPDepositorInsertSOBIR1
UseTagsYFPcExpressionPlantMutationPromoter35SAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
isoform PKC Epsilon (JCE592)
Plasmid#89776Purposeyeast expression of mouse PKC Epsilon with yeast PKC1 promoter and ADH1 terminatorDepositorInsertPKC Epsilon (Prkce Mouse)
UseTagsExpressionMammalianMutationPromoteryeast PKC1 promoter (-600 to -1)Available sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
p35S::SISOBIR1-YFPn
Plasmid#86163PurposeSOBIR1 split YFPDepositorInsertSOBIR1
UseTagsYFPnExpressionPlantMutationPromoter35SAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWHI2-HYG
Plasmid#80589PurposeCEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoterDepositorInsertWHI2 (WHI2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterWHI2 native promoterAvailable sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
His10-PS-SNAPf-9R (XSB525)
Plasmid#78513PurposeConstruct for expressing His10 - SNAPf -9R in E. ColiDepositorInsertSNAPf
UseTagsHis10 followed by a prescission protease claevage…ExpressionBacterialMutation9R added to the C-terminal of SNAPfPromoterAvailable sinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Scmh1-3'UTR
Plasmid#61785PurposeLuciferase reporter containing the 3'UTR of mouse Scmh1DepositorInsertmouse Scmh1 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Ywhab-3'UTR
Plasmid#61782PurposeLuciferase reporter containing the 3'UTR of mouse YwhabDepositorInsertmouse Ywhab 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Map3k1-3'UTR
Plasmid#61776PurposeLuciferase reporter containing the 3'UTR of mouse Map3k1DepositorInsertmouse Map3k1 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Crtap-3'UTR
Plasmid#61772PurposeLuciferase reporter containing the 3'UTR of mouse CrtapDepositorInsertmouse Crtap 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPMtb (GB0080)
Plasmid#68167PurposeProvides the S. Lycopersicum metallothionein II-like protein (MTB) gene promoter as a level 0 GoldenBraid partDepositorInsertSlMtb promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tg 2 kb Violet opsin GFP
Plasmid#72921Purposeviolet opsin promoter from zebra finch (Taeniopygia guttata) gDNA (nucleotides21,946 to 145, corresponding to chr1A_ran-dom:206453–208444 in taeGut1) driving GFPDepositorInsertviolet opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tg 2 kb Violet opsin DsRed
Plasmid#72920Purposeviolet opsin promoter from zebra finch (Taeniopygia guttata) gDNA (nucleotides21,946 to 145, corresponding to chr1A_ran-dom:206453–208444 in taeGut1) driving DsRedDepositorInsertviolet opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPAt2S3 (GB0029)
Plasmid#68162PurposeProvides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid partDepositorInsertAt2S3 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
miR-451 Trap
Plasmid#61487Purposeto knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoterDepositorInsertmiR-451 Trap
UseLentiviralTagsGFPExpressionMammalianMutationPromoterbidirectional phosphoglycerate kinase promoter (b…Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO
Plasmid#68356Purpose-The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoterDepositorInserts4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
FlpO recombinase
UseCRISPRTagsmTurquoise2 (BFP)ExpressionMammalianMutationPromoterSynthetic Probasin ARRx2 and U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
NET31 mRFP-N2 (490)
Plasmid#61984Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET31 (TMEM209 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIhh4.5k-Luc
Plasmid#53603PurposeReporter construct containing a 4.5 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Fhod1-3'UTR
Plasmid#61773PurposeLuciferase reporter containing the 3'UTR of mouse Fhod1DepositorInsertmouse Fhod1 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Tob1-3'UTR
Plasmid#61774PurposeLuciferase reporter containing the 3'UTR of mouse Tob1DepositorInsertmouse Tob1 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only