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Plasmid#107105PurposeLentiviral vector that codes for FLAG-RAB35 Q67L (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.DepositorInsertRAB35 (RAB35 Human)
UseLentiviralTagsFLAG tagExpressionMammalianMutationQ67LPromoterCMVAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLENTI6.3/AR-GC-E2325
Plasmid#85128Purposeexogenous AR expressionDepositorInsertandrogen receptor (AR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX_305_Ovalbumin
Plasmid#184924PurposeLentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.DepositorInsertOvalbumin (OVAL Chicken)
UseLentiviralTagsExpressionMammalianMutationOur vector has an A188T mutation that does not im…PromoterPGKAvailable sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)
Plasmid#196702PurposePlasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.DepositorInserthuman RNaseH1 wild type
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
phCMV-coGALVenv-C4070A
Plasmid#172217PurposeExpresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectorsDepositorInsertEnvelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
UseTagsExpressionMammalianMutationCarries C-terminus of amphotropic MLV strain 4070…PromoterCMV immediate early promoterAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flpo
Plasmid#55637PurposeExpresses Flpo in Mammalian CellsDepositorHas ServiceAAV Retrograde, AAV1, and AAV2InsertFlpo
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-vCre
Plasmid#55638PurposeExpresses vCre in Mammalian CellsDepositorHas ServiceAAV Retrograde and AAV8InsertvCre
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Cre
Plasmid#55636PurposeExpresses Cre in Mammalian CellsDepositorHas ServiceAAV RetrogradeInsertCre
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP
Plasmid#162337PurposeLentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6; mU6Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP
Plasmid#35511PurposeAAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsEYFPExpressionMammalianMutationE123T and T159CPromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-Ago2
Plasmid#74231PurposeGFP-Ago2 lentiviral vectorDepositorInsertAgo2 (AGO2 Human)
UseLentiviralTagseGFPExpressionMutationPromoterAvailable sinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry
Plasmid#35512PurposeAAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsmCherryExpressionMammalianMutationE123T and T159CPromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NurRE3
Plasmid#208253PurposepGL3 promoter luciferase reporter vector containing 3 copies of the NurRE POMC promoter DNA response element (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter assays in mammalian cellsDepositorInsert3X Nur response element LUC
UseTagsNoExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-HA
Plasmid#61355Purposeencodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal HA tag
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE
Plasmid#131003Purposebicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of CamKIIa promoterDepositorInsertChRmine
UseAAVTagsKv2.1-HAExpressionMutationPromoterCaMKIIaAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-VP64
Plasmid#47107PurposeExpresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cellsDepositorInsertdCas9-VP64
UseCRISPRTagsFlag, HA, SV40 NLS, and VP64ExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pE-35S-miniSdd7-nCas9-PBE-GmPPO2
Plasmid#204855PurposeFor cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)DepositorInsertminiSdd7-nSpCas9(D10A)-UGI-mScarlet
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9Promoter35S, AtU6-26pAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP
Plasmid#35501PurposeAAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.DepositorInserthChR2
UseAAVTagsEYFPExpressionMammalianMutationC128S and D156APromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pECE M2-SH3PXD2A WT
Plasmid#69813PurposeExpresses M2-SH3PXD2A in mammalian cellsDepositorInsertSH3 and PX domain-containing protein 2A (SH3PXD2A Human)
UseTagsFLAGExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-NEUROD1-T2A-PuroR
Plasmid#162338PurposeLentiviral expression of NEUROD1 under the control of the TetON promoterDepositorInsertNEUROD1 (NEUROD1 Human)
UseLentiviralTagsT2A-PuroRExpressionMammalianMutationPromoterTet-ONAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only