-
Plasmid#68937PurposeElastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residueDepositorInsertI48 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMito-mCherry-FRB_IRES_FKBP(I)-GBPen
Plasmid#128267PurposeBicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 1x FKBP-GBPenhancer (GFP Nanobody with 1 FKBP domain).DepositorInsertsMitoTrap
FKBP(I)-GBPen
UseTagsExpressionMammalianMutationPromoterCMV and IRESAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHA1.1-MFSD12-V5
Plasmid#149508PurposeStable expression of human MFSD12 with recoded nucleotide sequence.DepositorInsertMFSD12 (MFSD12 Human)
UseLentiviralTagsV5ExpressionMutationCodon Optimized SequencePromoterUbcAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-FGE
Plasmid#75147PurposeExpression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.DepositorInsertFGE
UseTagsHistidine tag and ThrombinExpressionBacterialMutationN/APromoterT7Available sinceJuly 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEasyCas9
Plasmid#184909PurposeExpresses the Streptococcus pyogenes Cas9 proteinDepositorInsertCas9
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCytERM_mScarlet-i_N1
Plasmid#85068PurposeIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)DepositorInsertCytERM
UseTagsmScarlet-iExpressionMammalianMutationaa1-29PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PM-FRB-CFP
Plasmid#67517PurposeFRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)DepositorInsertmTOR (MTOR Human)
UseTagsCFP, HA, and PM tethering sequence (MLCCMRRTKQVEK…ExpressionMammalianMutationFRB domain only (residues 2019–2114)PromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-flex-GFP-4x6T
Plasmid#196418PurposeCre-dependent AAV expression of GFP preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorInsertGFP
UseAAV and Cre/Lox; Astrocyte-selectiveTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
HsCLYBL-FLAG
Plasmid#111290Purposemammalian expressionDepositorInserthuman CLYBL (CLYBL Human)
UseTagslinker and FLAG tag: TGGGSGGSGGSMDYKDDDDKExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1
Plasmid#169449Purposeexpression of TRF1-mcherrry and sgRNA targeting telomeresDepositorInsertmCherry-TRF1
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
KRAB-dSpCas9
Plasmid#205248PurposeIn vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.DepositorInsertKRAB-dSpCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_hph
Plasmid#184912PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYCL_P2A_Hygro_Barcode
Plasmid#120462PurposeBarcoded lentiviral vector to express MYCL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYCL (MYCL Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-OTUB1 C91S-pcDNA3.1
Plasmid#118210PurposeExpresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cellsDepositorInsertOTUB1 (OTUB1 Human)
UseTags3x FLAGExpressionMammalianMutationchanged cysteine 91 to serinePromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Grp1-PH pEGFP-C1
Plasmid#71378PurposeFor analysis of PH domain localization in mammalian cellsDepositorInsertGrp1-PH (Cyth3 Mouse)
UseTagsEGFPExpressionMammalianMutationPleckstrin homology (PH) domain; aa 247-399PromoterCMVAvailable sinceNov. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCD86-EGFP
Plasmid#133858PurposeMammalian expression of CD86 C-terminally tagged with EGFPDepositorInsertCD86 (CD86 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRV∆L-5Cre
Plasmid#182964PurposeThe 3rd generation of rabies vector expressing Cre recombinaseDepositorInsertCRE recombinase
UseRabiesTagsExpressionMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-DDX4LCD
Plasmid#204408PurposeE. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tagDepositorInsertDDX4(1-236) (DDX4 Human)
UseTags6x-His; mCherryExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-4082NES(ForAMPK)
Plasmid#170310PurposeThe lentiviral vector for AMPK FRET-biosensorDepositorInsert4082NES (FRET biosensor for AMPK activity)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aalb_743
Plasmid#176662PurposeExpression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only