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Showing: 4821 - 4840 of 5223 results

  5. pH-ABE8e

    Plasmid
    #200774
    Purpose
    For plant adenine base editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038
    Insert
    nCas9(D10A)-TadA8e
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…
    Promoter
    maize Ubiquitin-1, OsU3
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200774-plasmid-map-sequence-id-399597

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  13. pUDP012

    Plasmid
    #101167
    Purpose
    E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101167-plasmid-map-sequence-id-199268

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  14. 2xFKBP-smFP(myc)-KDEL

    Plasmid
    #173021
    Purpose
    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    2xFKBP-smFP(myc)-KDEL
    Use
    Tags
    FKBP and smFP(myc) non-fluorescent
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    173021-plasmid-map-sequence-id-339210

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  16. pSOMA-T679A-NLS

    Plasmid
    #118938
    Purpose
    Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana
    Depositor
    Patrick Krysan
    Article
    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1
    Insert
    SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    118938-plasmid-map-sequence-id-232110

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  18. VRQR-HF1 variant

    Plasmid
    #138563
    Purpose
    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRQR-HF1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138563-plasmid-map-sequence-id-274789

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Showing: 4821 - 4840 of 5223 results