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Plasmid#161945PurposeContains Type IIS restriction site free elements for IBM staging vectors. Staging plasmid for IBM on mCherryDepositorInsertpSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G06
Plasmid#67140PurposeGateway destination vector for cell-free expression with C-term mCherry 8xHis tagDepositorTypeEmpty backboneUseCell-free expressionTagsHIS and mCherryExpressionMutationPromoterAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB332
Plasmid#68660PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB416
Plasmid#68681PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged tdTomato (tdTo…ExpressionPlantMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G09
Plasmid#67143PurposeGateway destination vector for cell-free expression with N-term 8xHis sfGFP tagDepositorTypeEmpty backboneUseCell-free expressionTagsHis and sfGFPExpressionMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP120-E3_5-HilA
Plasmid#153326PurposeTriggers secretion of SptP120 fused to the E3_5 DARPin from Salmonella upon induction with arabinoseDepositorInsertSptP120-E3_5 DARPin
UseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBP-pET-RBS
Plasmid#72981PurposePET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)DepositorInsertpET RBS derived from pET vectors
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM408
Plasmid#84132Purposetarget miRNA408 for destruction in Arabidopsis thalianaDepositorInsertSTTM408
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM398
Plasmid#84124Purposetarget miRNA398 for destruction in Arabidopsis thalianaDepositorInsertSTTM398
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM5026
Plasmid#84192Purposetarget miRNA5026 for destruction in Arabidopsis thalianaDepositorInsertSTTM5026
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pReporter_10
Plasmid#60570PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_10
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046931
Plasmid#145706PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pACBB-TetT-LVA
Plasmid#32555DepositorInsertTetR
UseSynthetic BiologyTagsLVA tagExpressionBacterialMutationPromoterConsitutive lacAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB436
Plasmid#68701PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE5
Plasmid#79461PurposesgRNA shuttle vector; module 1DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB231
Plasmid#68622PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsEmerald Luc fused with PEST sequence [ELuc(PEST)]ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.2 V5-DEST mir-1-1 reporter
Plasmid#46646Purposeexpression of query pri-miRNADepositorInsertmir-1-1 reporter
UseRNAi; Expression of query pri-mirna sequences in …TagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP120-K27-HilA
Plasmid#153328PurposeTriggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinoseDepositorInsertSptP120-K27 DARPin
UseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-013
Plasmid#78701PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only