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Plasmid#194220PurposeBackbone for constructing single casette expression plasmids using the Mammalian TookitDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only
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MTK Lentivirus Multi Cassette Destination Vector (pAN2424)
Plasmid#194221PurposeBackbone for constructing multi casette expression plasmids using the Mammalian TookitDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
LCB1_v1.3 (MTK 3a) (pZYW044)
Plasmid#194227PurposeMammalian Toolkit part 3a encoding the synthetic Spike binder LCB1DepositorInsertLCB1_v1.3
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
LCB3_v1.2 (MTK 3a) (pZYW045)
Plasmid#194228PurposeMammalian Toolkit part 3a encoding the synthetic Spike binder LCB3DepositorInsertLCB3_v1.2
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
WPRE (MTK 4b) (pAN2410)
Plasmid#194235PurposeMammalian Toolkit Part 4b encoding the WPRE for use with lentiviral backbonesDepositorInsertWPRE terminator
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
MTK234_043
Plasmid#123913PurposeEncodes the spCas9 pSV40 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA-pSV40
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK234_013
Plasmid#123989PurposeEncodes the spCas9 sgRNA1 hROSA26 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInserthROSA26 sg2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_031
Plasmid#123911PurposeEncodes the spCas9 sgRNA1 hCLYBL (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA2 -hCLYBL
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK2_025
Plasmid#123717PurposeEncodes crippled human pEF1alpha as a Type 2 part to be used in the MTK systemDepositorInsertpEF1ac
UseTagsExpressionMammalianMutationCrippled Kozac sequencePromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK2_003
Plasmid#123698PurposeEncodes pUAS (miniSV40 core) as a Type 2 part to be used in the MTK systemDepositorInsertpUAS (mini SV40)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pMA122 - peel-1 negative selection
Plasmid#34873PurposeNegative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegansDepositorInsertpeel-1 (peel-1 Nematode)
UseWorm targetingTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSCL.140
Plasmid#184995PurposeExpress Cas9 in human cellsDepositorInsertSpCas9
UseTagsSV40NLSExpressionMammalianMutationPromoterTetOn-3gAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hNox5
Plasmid#69354Purposeexpresses human Nox5 in mammalian cellsDepositorInserthNox5 (NOX5 Human)
UseTagsnoExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZmU3-gRNA
Plasmid#53061PurposeTranscription of guide RNA (gRNA) in maize cellsDepositorInsertzmU3 promoter - gRNA
UseCRISPR; Plant expressionTagsExpressionMutationPromotermaize U3Available sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
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C1C2CMUNC2C
Plasmid#218674PurposeInsect cell expression of a rat MUNC13-1 fragment containing domains C1C2BMUNC2CDepositorInsertMUNC13 (Unc13a Rat)
UseTagsGSTExpressionInsectMutationAmino acids1407-1453 deletedPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-CeSmg7_S
Plasmid#147465PurposeMammalian Expression of CeSmg7DepositorInsertCeSmg7 (smg-7 Nematode)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only