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Plasmid#213010PurposeTo study calcium signaling in AstrocytesDepositorInsertGfaABC1D-jGCaMP8m
UseAAVTagsExpressionMutationPromoterGfaABC1DAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-Lck-GFP
Plasmid#177333PurposeExpresses GFP on the plasma membrane of neurons via an Lck localization sequenceDepositorInsertLck-GFP
UseAAVTagsGFPExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
UPB-duodenum (UPB6)
Plasmid#180785PurposeUnique Projection Barcode (UPB6)-Duodenum for Projection-seqDepositorInserthM4D(Gi) (partial )
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D -IgK-TurboID-HA
Plasmid#219810PurposeSecreted HA-tagged TurboID for extracellular proximity labelingDepositorInsertIgK (Igk Mouse)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSaCas9-sgRNA-Tetra-com vector
Plasmid#131227PurposeFor expressing gRNA, whose Tetraloop is replaced with a com aptamerDepositorInsertvector for com modified sgRNA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRB_LD 0_CS G_tTA-BFP
Plasmid#58876PurposeMESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
V2-MESA-35F-M-tTA
Plasmid#84502PurposeMESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and tTADepositorInsertV2-MESA-35F-M-tTA
UseAAVTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMVP-Cas9FL-P2A-turboGFP
Plasmid#80941PurposePlasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP.DepositorInsertCas9FL
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
V1-MESA-45F-M-tTA
Plasmid#84500PurposeMESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and tTADepositorInsertV1-MESA-45F-M-tTA
UseAAVTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
flex-ChrimsonR-EYFP-Kv
Plasmid#135319PurposeCre-dependent expression of soma-targeted ChrimsonRDepositorInsertChrimsonR
UseAAVTagsEYFPExpressionMutationPromotersynapsinAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Rap-MESA-20-M-Gal4
Plasmid#84554PurposeMESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivatorDepositorInsertRap-MESA-FRB-20-M-Gal4
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
U6-Nme2_tracr
Plasmid#192146PurposeExpresses Nme2-sgRNA cloned with BspQIDepositorInsertNme2 sgRNA scaffold
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSMVP-Cas9C-P2A-turboGFP
Plasmid#80935PurposePlasmid that expresses Cas9C in mammalian cells; co-expresses turboGFP.DepositorInsertCas9C
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-p65(100-261)-RTA(125-190)
Plasmid#99673PurposeExpresses dSp Cas9 fused to combination of truncated activation domainsDepositorArticleInsertdCas9
UseTagsVP64-p65(101-261)-RTA(125-190)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Cyto-GCaMP3
Plasmid#44331DepositorInsertGCaMP3
UseAAV; Adeno-associated virus ()TagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
UPB-pancreas (UPB7)
Plasmid#180787PurposeUnique Projection Barcode (UPB7)-Pancreas for Projection-seqDepositorInserthM4D(Gi) (partial )
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Blat-BSD
Plasmid#163791PurposeThe plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.DepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
cPE
Plasmid#183549PurposeExpresses compact Prime Editor in mammalian cellsDepositorInsertSpCas9 nickase (H840A), Reverse Transcriptase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Lck-GCaMP3
Plasmid#44330DepositorInsertLck-GCaMP3
UseAAV; Adeno-associated virus ()TagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
GluR2_adRNA(1,20,6)-ADAR2
Plasmid#124620PurposeExpresses adRNA (1,20,6) targeting the RAB7A transcript along with the ADAR2DepositorInsertADAR2
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only