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Plasmid#207525PurposeConditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpRDepositorInsertoriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP;
UseCRISPR; Pseudomonas vectorTagsExpressionMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXPA10-NLS-3xmCitrine
Plasmid#192523PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertEXPANSIN A10 (EXPA10 Mustard Weed)
UseTags3xmCitrine and NLSExpressionPlantMutationPromoterEXPA10Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEVA424::tsi5
Plasmid#192960PurposeExpresses the protein Tsi51-76 for biological function studies in a broad-spectrum of organismsDepositorInserttsi5
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29a(+)::D1141A
Plasmid#192963PurposeTest the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGLDepositorInsertTse5_D1141A
UseTags9xhis + TEV tagExpressionBacterialMutationD1141APromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29a(+)::9xhis-Tse5
Plasmid#192962PurposeHeterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CTDepositorInsertTse5
UseTags9xhis + TEV tagExpressionBacterialMutationPromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPC030.306
Plasmid#171151PurposeMevalonate production pathway with J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-mvaES
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J3-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTEF1-UDH
Plasmid#166679PurposeYeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).DepositorInsertUDH
UseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF1Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTAMACHis6_PiuD
Plasmid#128953Purposeexpresses PiuD in E.coliDepositorInsertPutative outer membrane receptor for iron transport (PiuD)
UseTags6xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-105)PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b+R3(R112C/T180C)C
Plasmid#108194PurposeExpresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coliDepositorInsertPdR-PCNA3(R112C/T180C)-P450cam fusion protein
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b+3(R112C/T180C)C
Plasmid#108193PurposeExpresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coliDepositorInsertPCNA3(R112C/T180C)-P450cam fusion protein
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+2(L171C)X
Plasmid#108192PurposeExpresses PdR-PCNA2(L171C)-PdX fusion protein in E. coliDepositorInsertPCNA2(L171C)-PdX fusion protein
UseTagsHis6 tagExpressionBacterialMutationL171C (PCNA2); C73S/C85S (PdX)PromoterT7 promoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+R2(L171C)X
Plasmid#108191PurposeExpresses PdR-PCNA2(L171C)-PdX fusion protein in E. coliDepositorInsertPdR-PCNA2(L171C)-PdX fusion protein
UseTagsHis6 tagExpressionBacterialMutationL171C (PCNA2); C73S/C85S (PdX)PromoterT7 promoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+C3(R112C/T180C)X
Plasmid#108196PurposeExpresses P450cam-PCNA3(R112C/T180C)-PdX fusion protein in E. coliDepositorInsertP450cam-PCNA3(R112C/T180C)-PdX fusion protein
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3); C73S/C85S (PdX)PromoterT7 promoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+1(G108C)X
Plasmid#108189PurposeExpresses PCNA1(G108C)-PdX fusion protein in E. coliDepositorInsertPCNA1(G108C)-PdX fusion protein
UseTagsHis6 tagExpressionBacterialMutationG108C (PCNA1); C73S/C85S (PdX)PromoterT7 promoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS427-pCSDest2-AcrE2
Plasmid#85677PurposeMammalian expression of anti-CRISPR protein from Type I-EDepositorInsertType I-E anti-CRISPR
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC3R112C/T180C
Plasmid#85088PurposeExpresses PCNA3 variant (R112C/T180C) fused to P450cam (CYP101A1) in E. coliDepositorInsertThe R112C/T180C variant of PCNA3 fused to P450cam
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C in PCNA3PromoterT7 promoterAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVRa35_3582
Plasmid#49695DepositorInsertECF35_3582
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRc16_973
Plasmid#49737DepositorInsertAS16_973
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa07_1134
Plasmid#49660DepositorInsertECF07_1134
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
P20_992-TetR
Plasmid#97094PurposeExpresses TetR in the presence of Pseudomonas fluorescence sigma factor ECF20_992DepositorInsertTetR
UseTagsExpressionBacterialMutationPromoterP20_992Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only