Addgene

We narrowed to 683 results for: pcaggs

Showing: 461 - 480 of 683 results

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pCAG-nDsRedFL-miRNT

Plasmid

#177808

Purpose

A nontargeting miRNA based on the pre-miR- 30a structure (Qiu et al., 2008) has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-NT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-ChR2-mRuby2-ST

Plasmid

#109125

Purpose

Cation channelrhodopsin ChR2 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ChR2-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-mWasabi-ER

Plasmid

#164165

Purpose

Expresses mWasabi fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoter

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mWasabi

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-VAPB(1- 218)- eMagB

Plasmid

#162255

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

TagRFP-T-VAPB(1- 218)- eMagB

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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SETMAR-Cas9

Plasmid

#186701

Purpose

Plasmid encoding SETMAR-Cas9 fusion under CMV promoter

Depositor

Jennifer Doudna

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

Insert

SETMAR-Cas9

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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SETD2(SET)-Cas9

Plasmid

#186700

Purpose

Plasmid encoding SETD2-Cas9 fusion under CMV promoter

Depositor

Jennifer Doudna

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

Insert

SETD2(SET)-Cas9

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-nirButterfly

Plasmid

#136590

Purpose

Expression of a genetically-encoded voltage indicator nirButterfly

Depositor

Vladislav Verkhusha

Article

Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

Insert

nirButterfly

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G13 mutant

Plasmid

#231915

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1158 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al npj Viruses. 2025 Jan; 3(4). doi: 10.103

Insert

spike G13 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1158Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G14 mutant

Plasmid

#231916

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1173 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al npj Viruses. 2025 Jan; 3(4). doi: 10.103

Insert

spike G14 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1173Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G15 mutant

Plasmid

#231917

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1194 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al npj Viruses. 2025 Jan; 3(4). doi: 10.103

Insert

spike G15 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1194Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G1 mutant

Plasmid

#231913

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al npj Viruses. 2025 Jan; 3(4). doi: 10.103

Insert

spike G1 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G12 mutant

Plasmid

#231914

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1134 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al npj Viruses. 2025 Jan; 3(4). doi: 10.103

Insert

spike G12 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1134Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-CsChR-mRuby2-ST

Plasmid

#109130

Purpose

Cation channelrhodopsin CsChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

CsChR-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pPickles-2.31

Plasmid

#92336

Purpose

FRET-based biosensor for the measurement of BCR-ABL activity

Depositor

Yusuke Ohba

Article

Mizutani et al Clin Cancer Res. 2010 Aug 1;16(15):3964-

Insert

Pickles-2.31

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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Ac 1.8kb red opsin GFP.

Plasmid

#72915

Purpose

red opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving GFP

Depositor

Joseph Corbo

Article

Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

Insert

red opsin promoter

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Ac 1.8kb red opsin DsRed

Plasmid

#72914

Purpose

red opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving DsRed

Depositor

Joseph Corbo

Article

Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

Insert

red opsin promoter

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Gg 1.8kb rhodopsin GFP

Plasmid

#72917

Purpose

Rhodopsin promoter from chicken (Gallus gallus) gDNA chr12:19,499,638–19,501,514 in galGal4 driving GFP

Depositor

Joseph Corbo

Article

Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

Insert

chicken rhodopsin (RHO Chicken)

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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Gg 1.8kb rhodopsin DsRed

Plasmid

#72916

Purpose

Rhodopsin promoter from chicken (Gallus gallus) gDNA chr12:19,499,638–19,501,514 in galGal4 driving DsRed

Depositor

Joseph Corbo

Article

Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

Insert

chicken rhodopsin (RHO Chicken)

Use

Tags

Expression

Mutation

Promoter

rhodopsin

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-mRuby3

Plasmid

#171154

Purpose

Encodes mRuby3 fluorophore

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

mRuby3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pJRH-1179 U6-reci Gag-Cas9 v2

Plasmid

#201914

Purpose

Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-Cas9 v2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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