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Plasmid#205067PurposeMTF1 protein expressionDepositorInsertMTF1 (MTF1 Budding Yeast)
UseTagsExpressionBacterialMutationLacks C-terminal 9 amino acidsPromoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AV13_pCAG.Cas9.gRNA.NT
Plasmid#199260PurposeExpression construct encoding SpCas9 nuclease and a control non-targeting guide RNADepositorInsertsSpCas9 nuclease
Non-targeting (NT) control gRNA
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterCAG promoter and RNA polymerase III promoter for …Available sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggybac-EF1-halo-Oct4
Plasmid#197909Purposesingle-particle tracking for OCT4DepositorInsertpou5f1 (Pou5f1 Mouse)
UseTagsHalotagExpressionMammalianMutationPromoterEF1Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-Sec22b
Plasmid#168029PurposeExpresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cellsDepositorInserteCFP-Sec22b(A131-H190)-mVenus
UseTagsHisx6 and mycExpressionBacterialMutationPromoterAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-VAMP8
Plasmid#168028PurposeExpresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cellsDepositorInserteCFP-VAMP8(G7-R67)-mVenus
UseTagsHisx6 and mycExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_2_lacZ
Plasmid#155173Purposesee pDUAL_lacZ: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_2_tacI
Plasmid#155174Purposesee pDUAL_tacI: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBICI_2_medium
Plasmid#155167Purposesee pBICI_medium: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAL928
Plasmid#119853Purposeprecusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427CDepositorInsertprecursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
UseTagsnoneExpressionBacterialMutationCysteine 169 to Serine, Cysteine 179 to Serine, C…PromoterT7Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(bxb)_lox
Plasmid#183762PurposeBxb1-specific donor plasmid for the cloning of payloads <20 kbDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
LZ10 PBREBAC-H2BHalo
Plasmid#91564Purposepiggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.DepositorInserthistone H2B-HaloTag
UseTagsHaloTagExpressionMammalianMutationPromoterEF1Available sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEFBOS_CreIRESpuro
Plasmid#183812PurposeExpresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoterDepositorInsertCre recombinase
UseSynthetic BiologyTagsNLSExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-Bxb1-LP-v2-TC
Plasmid#194327PurposeCLYBL targeting construct with the Bxb1 landing pad version 2 cassette.DepositorInsertloxP-EBFP2-attP-BleoR-lox251
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
YE1-BE4max-NG
Plasmid#138159PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 D10A + …PromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(C31)_FRT
Plasmid#183761PurposephiC31-specific donor plasmid for the cloning of payloads <20 kbDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Bxb1-LP-v2-TC
Plasmid#194326PurposeAAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.DepositorInsertloxP-EBFP2-attP-BleoR-lox251
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS
Plasmid#199252PurposeExpression construct encoding the high specificity eSpCas9(1.1)-D10A nickaseDepositorInsertHigh specificity eSpCas9(1.1)-D10A nickase
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10A K848A K1003A R1060APromoterCAG promoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM4787
Plasmid#120088PurposeCyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).DepositorInsertPlasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMB-SaCas9
Plasmid#105998PurposeSaCas9 expression vectorDepositorInsertCas9 nuclease
UseCRISPR and LentiviralTagsnoneExpressionMammalianMutationnonePromotershort EF1alphaAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Bxb1_miRFP703
Plasmid#183759PurposeBxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703DepositorInsertmiRFP703
UseTagsLck lipidation signalExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only