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Plasmid#158151PurposeConstruction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only
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pX330S-5
Plasmid#58781PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-BFP-CAAX
Plasmid#184459PurposePlasma membrane-targeted EBFP2 expression in mammalian cellsDepositorInsertEBFP2-CAAX
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-BFP-NLS
Plasmid#184458PurposeNucleus-targeted EBFP2 expression in mammalian cellsDepositorInsertEBFP2-3xNLS
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-2XboxB
Plasmid#75391PurposesgRNA1-2XboxBDepositorInsertsgRNA1-2XboxB
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ145
Plasmid#69297PurposeGolden Gate recipient and Gateway entry vector; assembly of 5 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTns(Ptr)
Plasmid#175576PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTnsABC
UseTagsExpressionBacterialMutationNOPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDonorCm(Ptr)
Plasmid#175577PurposeDNA donor for transpositionDepositorInsertMini-Tn, Cm cassette
UseTagsExpressionBacterialMutationNOPromoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_HaloTag9_LamB1
Plasmid#175538PurposeExpression of HaloTag9 at the nuclear lamina of the mammalian cellsDepositorInsertHaloTag9-LamB1
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x4
Plasmid#58768PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ148
Plasmid#69300PurposeGolden Gate recipient and Gateway entry vector; assembly of 8 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOS009: QuasAr2-mOrange2 voltage sensor (plasma membrane)
Plasmid#163043PurposeQuasAr2-mOrange2 voltage sensor (plasma membrane)DepositorInsertQuasAr2
UseLentiviralTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_LAMP1_HaloTag9
Plasmid#175541PurposeLyzosomal expression of HaloTag9 in mammalian cellsDepositorInsertLAMP1-HaloTag9
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lifeact_HaloTag7
Plasmid#175535PurposeExpression of HaloTag7 at the actin filaments of mammalian cellsDepositorInsertLifeact-HaloTag7
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_CalR_HaloTag7_KDEL
Plasmid#175530PurposeExpression of HaloTag7 in the endoplasmid reticulum of mammalian cellsDepositorInsertCalR-HaloTag7-KDEL
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF_dCas9
Plasmid#68416PurposeTransient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.DepositorInsertdCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A/H841APromoterhuman EF1[alpha]Available sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGGDestTol2LC-3sgRNA
Plasmid#64241Purposedestination vector for three U6x:sgRNA cassettesDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRU45
Plasmid#167667PurposeENTRY vector for gRNA5 cloning under pAtU6 promoterDepositorInsertpAtU6
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGGDestTol2LC-1sgRNA
Plasmid#64239Purposedestination vector for one U6x:sgRNA cassetteDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_LAMP1_HaloTag7
Plasmid#175531PurposeLyzosomal expression of HaloTag7 in mammalian cellsDepositorInsertLAMP1-HaloTag7
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only