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Plasmid#186386PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter emiRFP703 into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only
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pUPD2 hpIntron (GB1281)
Plasmid#160569PurposeIntron sequence from S. lycopersicumDepositorInserthpIntron
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKP1469
Plasmid#160815PurposeExpression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD)DepositorInsertexbB
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 MS2 (GB2665)
Plasmid#160597PurposeCDS of MS2 protein with B3 nomenclatureDepositorInsertMS2
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
FtsZ
Plasmid#74128PurposeExpresses FtsZ without a peptideDepositorInsertFtsZ
UseTagsC-terminal linker sequence (G G S G S G S S)ExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE
Plasmid#65821PurposeControl guide RNA plasmid E. coli , low phosphate inductionDepositorInsertcontrol guideRNA
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TOgw-C-mEGFP
Plasmid#223953PurposeGateway cloning of genes of interest with a C-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TOgw-C-mCherry
Plasmid#223952PurposeGateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TOgw-C-mECFP
Plasmid#223950PurposeGateway cloning of genes of interest with a C-terminal mECFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseTagsmECFPExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TOgw-C-mEYFP
Plasmid#223951PurposeGateway cloning of genes of interest with a C-terminal mEYFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseTagsmEYFPExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TOgw-N-mCherry
Plasmid#223948PurposeGateway cloning of genes of interest with an N-terminal mCherry fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TOgw-N-mEGFP
Plasmid#223949PurposeGateway cloning of genes of interest with an N-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-R_dummy-RBS
Plasmid#204081PurposeType 0 RBS partDepositorInsertDummy sequence
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-C_ccdB
Plasmid#204114PurposeType 0 CDS partDepositorInsertToxic DNA gyrase inhibitor ccdB
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-C_mTagBFP2
Plasmid#204109PurposeType 0 CDS partDepositorInsertmTagBFP2 blue fluorescent protein
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-C_dummy-CDS
Plasmid#204108PurposeType 0 CDS partDepositorInsertDummy sequence
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-P_dummy-promoter
Plasmid#204069PurposeType 0 promoter partDepositorInsertDummy sequence
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL2_213
Plasmid#210697PurposeEven ORI-marker screen destination vector; contains sfYFP dropout for barcoded-ORI insertionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_212
Plasmid#210696PurposeEven ORI-marker screen destination vector; contains sfYFP dropout for barcoded-ORI insertionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_211
Plasmid#210695PurposeEven ORI-marker screen destination vector; contains sfYFP dropout for barcoded-ORI insertionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only