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pRS315e_pGal-dCas9-PmCDA1

Plasmid

#79615

Purpose

Expresses dCas9-PmCDA1 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Use

Tags

PmCDA1

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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HiFi dCas9 KRAB

Plasmid

#188500

Purpose

Expresses FLAG tagged HiFi dCas9 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A/R691A/H840A

Promoter

EF1a

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMT1-24X-NOG(AtUBI10)

Plasmid

#202016

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

Inserts

dCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1

Use

Tags

GB1 and sfGFP

Expression

Plant

Mutation

Promoter

AtUBI10

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRH2502

Plasmid

#84379

Purpose

Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

Depositor

Robert Husson

Article

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

dcas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Aspartate at position 10 changed to Alanine, Hist…

Promoter

uv15tetO

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pEasyG1_hph

Plasmid

#184912

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-nCas9(D10A)-PmCDA1

Plasmid

#79617

Purpose

Expresses nCas9(D10A)-PmCDA1 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Use

Tags

PmCDA1

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pNHEJ-Cas9-recX

Plasmid

#158715

Purpose

CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

cas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

TetO

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pJIT163-2NLSCas9

Plasmid

#53064

Purpose

Expression of rice codon-optimized Cas9 in plant cells

Depositor

Caixia Gao

Article

Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

Insert

Cas9

Use

Plant expression

Tags

Expression

Mutation

Rice codon optimized

Promoter

2x35S

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCRISPRai

Plasmid

#189938

Purpose

Expresses CRISPRai cassette under EF1a promoter

Depositor

Yu-Chen Hu

Article

Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

Insert

CRISPRai cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV enhancer-rat EF1 alpha

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRB14

Plasmid

#52522

Purpose

expresses a myc-tagged version of hCas9 in Drosophila

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

S. pyogenes cas9 with humanized codon bias

Use

Tags

myc

Expression

Insect

Mutation

Promoter

tubulin

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pspCas9-HF-tetra-com-vector

Plasmid

#132555

Purpose

Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Inserts

High fidelity Cas9
sgRNA scaffold with com replacing the Tetraloop

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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AV44_pCAG.Cas9-D10A.gRNA.S1

Plasmid

#199258

Purpose

Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9-D10A nickase
AAVS1-targeting gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter and RNA polymerase III promoter for …

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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His-TEV-GB1 Leu5Met

Plasmid

#128686

Purpose

Expresses GB1 Leu5Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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AV15_pCAG.Cas9.gRNA.S1

Plasmid

#199259

Purpose

Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9 nuclease
AAVS1-targeting gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CAG promoter and RNA polymerase III promoter for …

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEasyG1_nat

Plasmid

#184911

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pKMD106h - intein-Cas9(C574)

Plasmid

#64191

Purpose

Expresses intein-Cas9(C574) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEasyG1_zeo

Plasmid

#184910

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-6-AtSpCas9-9

Plasmid

#197778

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AtSpCas9

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCT

Plasmid

#60620

Purpose

Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

Depositor

Huimin Zhao

Article

Bao et al ACS Synth Biol. 2014 Sep 19.

Inserts

iCas9
tracrRNA

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Yeast

Mutation

changed Aspartate 147 to Tyrosine, Proline 411 to…

Promoter

RPR1p and TEF1p

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pJT43_GalL_BE-P(AP)3

Plasmid

#145036

Purpose

Expresses BE-P(AP)3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE-(AP)3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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