Addgene

We narrowed to 2,242 results for: PRK

Showing: 461 - 480 of 2242 results

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PRKAR2B gRNA (BRDN0001147707)

Plasmid

#76152

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR2B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR2B (PRKAR2B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-RagC(L91P)

Plasmid

#99721

Purpose

Mammalian expression of HA-RagC(L91P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Use

Tags

HA

Expression

Mutation

L91P

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-RagA(R37P)

Plasmid

#99713

Purpose

Mammalian expression of HA-RagA(R37P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Use

Tags

HA

Expression

Mutation

R37P

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 Halo RagB

Plasmid

#112753

Purpose

express Halo-RagB

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Halo-RagB (RRAGB Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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R777-E201 Hs.PRKAG2

Plasmid

#70485

Purpose

Gateway ORF clone of human PRKAG2 [NM_024429.1] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

PRKAG2 (PRKAG2 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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PRKAA1 gRNA (BRDN0001147273)

Plasmid

#76254

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAA1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAA1 (PRKAA1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKAA2 gRNA (BRDN0001146900)

Plasmid

#76635

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAA2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAA2 (PRKAA2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Sestrin2-E451A

Plasmid

#73674

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-HA CASTOR2 ACT2

Plasmid

#84494

Purpose

overexpression of CASTOR2 ACT2 domain (aa 171-end)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 ACT2 (GATSL2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

171-last amino acid

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-HA CASTOR1 ACT2

Plasmid

#84492

Purpose

overexpression of CASTOR1 ACT2 domain (aa 169-end)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 ACT2 (GATSL3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

169-last amino acid

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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PRKD2 gRNA (BRDN0001147619)

Plasmid

#77615

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKD2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKD2 (PRKD2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKD3 gRNA (BRDN0001146689)

Plasmid

#75951

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKD3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKD3 (PRKD3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKD3 gRNA (BRDN0001146945)

Plasmid

#75953

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKD3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKD3 (PRKD3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-Sestrin2-L261A

Plasmid

#73672

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

L261A changed Leucine 261 to Alanine

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pRK5 Flag RagB (GD)

Plasmid

#112770

Purpose

express Flag-RagB (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagB (G domian only) (RRAGB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 GFP RagC (GD)

Plasmid

#112767

Purpose

express GFP-RagC (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagC (G domain only) (RRAGC Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagC (GD)

Plasmid

#112772

Purpose

express Flag-RagC (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (G domain only) (RRAGC Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 GFP RagB (GD)

Plasmid

#112765

Purpose

express GFP-RagB (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagB (G domain only) (RRAGB Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKCE gRNA (BRDN0001148049)

Plasmid

#76791

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCE

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCE (PRKCE Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKG1 gRNA (BRDN0001146382)

Plasmid

#77004

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKG1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKG1 (PRKG1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge