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Plasmid#202784PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H266A-stopDepositorInsertATG3 (ATG3 Human)
UseTags6xHis, TEVExpressionBacterialMutationH266APromoterAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 ATG3 WT
Plasmid#129292PurposeGateway entry clone encoding wild-type human ATG3 with a stop codonDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro GFP-GABARAPL1 G116
Plasmid#123243PurposeExpression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationDeleted amino acid 117. Stop codon after G116PromoterPGKAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hATG7wt
Plasmid#87867PurposeExpress a wild type human Atg7/Apg7 in mammalian cellsDepositorInsertATG7 (ATG7 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_10xHisTEVATG5_ATG12
Plasmid#190861PurposePlasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178DepositorUseTags6xHis-TEVExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
ReactionC_pBIG1a_ATG7_ATG10
Plasmid#190860PurposePlasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179DepositorUseTagsExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 C264A
Plasmid#129297PurposeExpresses 3xFLAG-ATG3 C264A in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged Cysteine 264 to AlaninePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY GBRPL1
Plasmid#89294PurposeFor expression of the mammalian Atg8 protein GABARAPL1 without a tagDepositorInsertGABARAPL1 (GABARAPL1 Human)
UseRetroviralTagsIRES-YFPExpressionMammalianMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hGATE-16
Plasmid#87870PurposeExpress a EGFP-human GATE-16 in mammalian cellsDepositorInsertGATE-16 (GABARAPL2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY HA-GBRPL2
Plasmid#89298PurposeFor expression of the mammalian Atg8 protein GABARAPL2 tagged with HADepositorInsertGABARAPL2 (GABARAPL2 Human)
UseRetroviralTagsHA and IRES-YFPExpressionMammalianMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY HA-GBRPL1
Plasmid#89297PurposeFor expression of the mammalian Atg8 protein GABARAPL1 tagged with HADepositorInsertGABARAPL1 (GABARAPL1 Human)
UseRetroviralTagsHA and IRES-YFPExpressionMammalianMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBMN mCherry-p62(W340A/ΔUBD)
Plasmid#119688PurposeFor mammalian expression of LIR mutant p62 without its UBDDepositorInsertp62 (SQSTM1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationW340A & UBD deletion (1-385)PromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 K243R
Plasmid#129301PurposeExpresses pCMV 3xFLAG-ATG3 K243R in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged Lysine 243 to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY GBRPL2
Plasmid#89295PurposeFor expression of the mammalian Atg8 protein GABARAPL2 without a tagDepositorInsertGABARAPL2 (GABARAPL2 Human)
UseRetroviralTagsIRES-YFPExpressionMammalianMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-muGFP-hATG2A mLIR
Plasmid#215506PurposeExpresses GFP tagged human ATG2A with the mutation in LC3 interacting region.DepositorInsertAutophagy related 2A (ATG2A Human)
UseRetroviralTagsmuGFPExpressionMammalianMutationP656R (natural variant with no functional relevan…PromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 ATG3 C264A
Plasmid#129293PurposeGateway entry clone encoding human ATG3 C264A (catalytic inactive)DepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationchanged Cysteine 264 to AlaninePromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 ATG3 K243R
Plasmid#129294PurposeGateway entry clone encoding human ATG3 K243RDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationchanged Lysine 243 to ArgininePromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 ATG3 allKR
Plasmid#129295PurposeGateway entry clone encoding human ATG3 with all 22 lysine residues mutated to arginineDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationchanged all 22 Lysine residues to ArgininePromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 allKR
Plasmid#129298PurposeExpresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged all 22 Lysine residues to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 K295R
Plasmid#129302PurposeExpresses pCMV 3xFLAG-ATG3 K295R in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged Lysine 295 to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only