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pDONR223 ATG3 WT

Plasmid

#129292

Purpose

Gateway entry clone encoding wild-type human ATG3 with a stop codon

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-hATG7wt

Plasmid

#87867

Purpose

Express a wild type human Atg7/Apg7 in mammalian cells

Depositor

Isei Tanida, Eiki Kominami

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

ATG7 (ATG7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBIG1b_10xHisTEVATG5_ATG12

Plasmid

#190861

Purpose

Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG5 (ATG5 Human)
ATG12 (ATG12 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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ReactionC_pBIG1a_ATG7_ATG10

Plasmid

#190860

Purpose

Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG7 (ATG7 Human)
ATG10 (ATG10 )

Use

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-ATG3 C264A

Plasmid

#129297

Purpose

Expresses 3xFLAG-ATG3 C264A in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Cysteine 264 to Alanine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMX-IY GBRPL1

Plasmid

#89294

Purpose

For expression of the mammalian Atg8 protein GABARAPL1 without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL1 (GABARAPL1 Human)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-hGATE-16

Plasmid

#87870

Purpose

Express a EGFP-human GATE-16 in mammalian cells

Depositor

Isei Tanida, Eiki Kominami

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

GATE-16 (GABARAPL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMX-IY HA-GBRPL2

Plasmid

#89298

Purpose

For expression of the mammalian Atg8 protein GABARAPL2 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Retroviral

Tags

HA and IRES-YFP

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMX-IY HA-GBRPL1

Plasmid

#89297

Purpose

For expression of the mammalian Atg8 protein GABARAPL1 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL1 (GABARAPL1 Human)

Use

Retroviral

Tags

HA and IRES-YFP

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-p62(W340A/ΔUBD)

Plasmid

#119688

Purpose

For mammalian expression of LIR mutant p62 without its UBD

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

p62 (SQSTM1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

W340A & UBD deletion (1-385)

Promoter

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Puro GFP-GABARAPL1 G116

Plasmid

#123243

Purpose

Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

PGK

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-ATG3 K243R

Plasmid

#129301

Purpose

Expresses pCMV 3xFLAG-ATG3 K243R in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Lysine 243 to Arginine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMX-IY GBRPL2

Plasmid

#89295

Purpose

For expression of the mammalian Atg8 protein GABARAPL2 without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IP-muGFP-hATG2A mLIR

Plasmid

#215506

Purpose

Expresses GFP tagged human ATG2A with the mutation in LC3 interacting region.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

Autophagy related 2A (ATG2A Human)

Use

Retroviral

Tags

muGFP

Expression

Mammalian

Mutation

P656R (natural variant with no functional relevan…

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223 ATG3 C264A

Plasmid

#129293

Purpose

Gateway entry clone encoding human ATG3 C264A (catalytic inactive)

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

changed Cysteine 264 to Alanine

Promoter

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 ATG3 K243R

Plasmid

#129294

Purpose

Gateway entry clone encoding human ATG3 K243R

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

changed Lysine 243 to Arginine

Promoter

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 ATG3 allKR

Plasmid

#129295

Purpose

Gateway entry clone encoding human ATG3 with all 22 lysine residues mutated to arginine

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

changed all 22 Lysine residues to Arginine

Promoter

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV 3xFLAG-ATG3 allKR

Plasmid

#129298

Purpose

Expresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed all 22 Lysine residues to Arginine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV 3xFLAG-ATG3 K295R

Plasmid

#129302

Purpose

Expresses pCMV 3xFLAG-ATG3 K295R in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Lysine 295 to Arginine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-hATG7CS

Plasmid

#87868

Purpose

Express a active-site mutant human Atg7/Apg7 C572S in mammalian cells

Depositor

Isei Tanida, Eiki Kominami

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

ATG7 C572S mutant (ATG7 Human)

Use

Tags

Expression

Mammalian

Mutation

C572S mutant

Promoter

CMV

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge