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Addgene
Showing: 461 - 480 of 752 results

  1. Venus-7aa-MAO-pEGFP-C3

    Plasmid
    #166765
    Purpose
    To target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    MAO tail anchor (MAOA Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    166765-plasmid-map-sequence-id-328859

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  2. pCAX-SCLM

    Plasmid
    #216757
    Purpose
    For strong and long-lasting expression of SuperClomeleon in mammalian cells from the hybrid CAG promoter
    Depositor
    George Augustine
    Insert
    SuperClomeleon
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    CAG (cytomegalovirus-chicken beta-actin-rabbit be…
    Availability
    Academic Institutions and Nonprofits only
    216757-plasmid-map-sequence-id-429395

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  3. pMTB-Multibow-C

    Plasmid
    #60979
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic cerulean
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    Cerulean
    Use
    Zebrafish
    Tags
    Expression
    Mutation
    Promoter
    Bactin2
    Availability
    Academic Institutions and Nonprofits only

  4. pMTB-Multibow-mC

    Plasmid
    #60986
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound cerulean
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    Cerulean
    Use
    Zebrafish
    Tags
    membrane tag
    Expression
    Mutation
    Promoter
    Bactin2
    Availability
    Academic Institutions and Nonprofits only

  5. pMTB-Multibow-hC

    Plasmid
    #60993
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear cerulean
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    Cerulean
    Use
    Zebrafish
    Tags
    histone 2B tag
    Expression
    Mutation
    Promoter
    Bactin2
    Availability
    Academic Institutions and Nonprofits only

  6. pCFP

    Plasmid
    #175593
    Purpose
    Lac inducible, AMP Resistant, cerulean gene on a low copy backbone. Used as a negative control to the pMesh1_CFP plasmid.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    CFP (cerulean)
    Use
    Tags
    Expression
    Mutation
    An improved monomeric CFP. See doi: 10.1038/nbt945
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175593-plasmid-map-sequence-id-348363

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  7. pAAV/CMV-SCLM

    Plasmid
    #216758
    Purpose
    AAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleon
    Depositor
    George Augustine
    Insert
    SuperClomeleon followed by WPRE and human growth hormone polyA terminator
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    CMV (cytomegalovirus)
    Availability
    Academic Institutions and Nonprofits only
    216758-plasmid-map-sequence-id-429489

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  8. pAAV/EF1a-DIO-SCLM

    Plasmid
    #216759
    Purpose
    AAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoter
    Depositor
    George Augustine
    Insert
    SuperClomeleon
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    EF1a (human elongation factor 1 alpha)
    Availability
    Academic Institutions and Nonprofits only
    216759-plasmid-map-sequence-id-429631

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  9. pAAV/hSyn-SCLM

    Plasmid
    #216760
    Purpose
    AAV2 transfer vector with the human synapsin I promoter for neuronal expression of SuperClomeleon
    Depositor
    George Augustine
    Insert
    SuperClomeleon
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    hSyn (human synapsin I)
    Availability
    Academic Institutions and Nonprofits only
    216760-plasmid-map-sequence-id-429492

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  10. Nemato

    Plasmid
    #24006
    Depositor
    Lawrence Cohen
    Insert
    VSFP
    Use
    Tags
    Cerulean and Citruline
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    24006-plasmid-map-sequence-id-11277

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  12. SuperClomeleon

    Plasmid
    #203724
    Purpose
    An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride
    Depositor
    George Augustine
    Article
    Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.
    Insert
    Cerulean linked topaz fluorescent protein
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Cerulean, modified Topaz Fluorescent Protein with…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203724-plasmid-map-sequence-id-403640

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  13. Zahra

    Plasmid
    #23958
    Depositor
    Lawrence Cohen
    Insert
    VSP (tpte Zebrafish)
    Use
    Tags
    Cerulean and Citrulline
    Expression
    Mammalian
    Mutation
    R153Q
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    23958-plasmid-map-sequence-id-11262

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  14. Zahra73-59

    Plasmid
    #23957
    Depositor
    Lawrence Cohen
    Insert
    VSP (tpte Zebrafish)
    Use
    Tags
    Cerulean and Citrulline
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    23957-plasmid-map-sequence-id-11261

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  15. Zahra76-77

    Plasmid
    #23959
    Depositor
    Lawrence Cohen
    Insert
    VSP (tpte Zebrafish)
    Use
    Tags
    Cerulean and Citrulline
    Expression
    Mammalian
    Mutation
    T156R
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    23959-plasmid-map-sequence-id-11263

    Enlarge

  16. Zahra78-79

    Plasmid
    #23960
    Depositor
    Lawrence Cohen
    Insert
    VSP (tpte Zebrafish)
    Use
    Tags
    Cerulean and Citrulline
    Expression
    Mammalian
    Mutation
    R153Q T156R
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    23960-plasmid-map-sequence-id-11264

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  18. Zahra74-75, 80-81

    Plasmid
    #23961
    Depositor
    Lawrence Cohen
    Insert
    VSP (tpte Zebrafish)
    Use
    Tags
    Cerulean and Citrulline
    Expression
    Mammalian
    Mutation
    R153Q I165R
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    23961-plasmid-map-sequence-id-11265

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Showing: 461 - 480 of 752 results