Addgene

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pscALPS-SIV-AGM-MAL vpr

Plasmid

#115828

Purpose

Encodes codon optimized 3xFLAG-SIVAGM Vpr (MAL_ZMB) (LC114462) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGM Vpr (MAL_ZMB) (LC114462)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pNIC28-BSA4-G-S10

Plasmid

#127828

Purpose

Production of recombinant human ribosomal protein S10

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS10 (RPS10 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry

Plasmid

#68280

Purpose

SKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherry

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Mkk2DS NLS mCherry (MKK2 Budding Yeast)

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

RPS2

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3B09

Plasmid

#70998

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Hrb87F (Hrb87F Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pAV-puro-U6+27-F30-Tornado-Squash-FTL-IRE

Plasmid

#216828

Purpose

Expresses a circular RNA containing a Squash aptamer and an IRE (FTL derived) in mammalian cells, using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Squash-FTL-IRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pJQR3

Plasmid

#218289

Purpose

Introduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6

Depositor

Claudia Vickers

Article

McDonnell et al bioRxiv 2024.03.26.586389

Insert

HO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036W

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pITEu2

Plasmid

#218282

Purpose

Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus

Depositor

Claudia Vickers

Article

Evans et al bioRxiv 2024.01.30.578080

Insert

HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJE13HD7

Plasmid

#218281

Purpose

Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.

Depositor

Claudia Vickers

Article

Evans et al bioRxiv 2024.01.30.578080

Insert

HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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RGG-BcLOV-mCh-EGFR

Plasmid

#208280

Purpose

Expresses RGG-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

RGG-BcLOV-mCh-EGFR

Use

Lentiviral

Tags

Expression

Mutation

H225N in mCherry- please see depositor comment

Promoter

pCMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO-ChemoL-NAD-NLS3x

Plasmid

#193828

Purpose

CMV overexpression of ChemoL-NAD in the nucleus of mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoL-NAD-NLS3x

Use

Tags

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BALF3

Plasmid

#195828

Purpose

Gateway Entry Clone of Epstein-Barr virus BALF3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BALF3

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-HseIF4E_36-217_AA

Plasmid

#148282

Purpose

Bacterial Expression of HseIF4E_36-217

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

HseIF4E_36-217 (EIF4E Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-dsRNAres_AA

Plasmid

#148288

Purpose

Insect Expression of DmCaf40-dsRNAres

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmCaf40-dsRNAres (Rcd-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Pm_MSP7.4-Cd4-bio-His

Plasmid

#126828

Purpose

Expression of recombinant P. malariae protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

MSP7.4 (PmUG01_12029900 Plasmodium malariae)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 Ub-NLRP1 L1215

Plasmid

#166828

Purpose

pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1215 N-terminus (no stop)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Ubiquitin

Expression

Mutation

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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D12_Ld_040370

Plasmid

#135828

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pET27b-ecDHFR-N23PP

Plasmid

#110828

Purpose

Bacterial expression of E.coli DHFR N23PP mutant

Depositor

Stephen Benkovic

Article

Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

Insert

ecDHFR

Use

Tags

Expression

Bacterial

Mutation

N23 mutated to P23; a proline residue inserted be…

Promoter

T7

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF2C05

Plasmid

#70891

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Orc2 (Orc2 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only
pUAST YFP Rab8 DN

Plasmid

#58451

Purpose

expression of drosophila YFP Rab8 dominant negative mutant

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab8 (Rab8 Fly)

Use

Tags

YFP

Expression

Insect

Mutation

T22N (dominant negative)

Promoter

GAL4

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf140

Plasmid

#12828

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jon Yewdell, Jack Bennink

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf140

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only