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pB6-Csrp2-T2A-sfGFP-iCre-ERT2-FNF-DTA

Plasmid

#140270

Purpose

B6J Csrp2-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number

Depositor

Mario Capecchi

Insert

Csrp2 (Csrp2 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

C-terminal fusion of T2A-sfGFP-iCre-ERT2

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCDB368

Plasmid

#110276

Purpose

gHH_44 with a N-terminal 10-His DsbC TEV-peptide fusion

Depositor

Christopher Bahl

Article

Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

Insert

gHH_44

Use

Tags

10-His, Tev recognition peptide, and mature DsbC …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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Tmem194 pEGFP-N2 (1074)

Plasmid

#62027

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem194 (TMEM194A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
RcSxph P727A

Plasmid

#194027

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

P727A

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pWR76

Plasmid

#202776

Purpose

episomal fluorescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCherry
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR23

Plasmid

#202770

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coKanMX
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR10

Plasmid

#202710

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coShBle
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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AA226

Plasmid

#216027

Purpose

Fragmid fragment: (Cas protein) Cas7-11

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas7-11_v1.1 [Di]

Use

CRISPR; Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFP-N

Plasmid

#210270

Purpose

N-terminal EGFP-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Type

Empty backbone

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pWR24

Plasmid

#202771

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coNAT
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pWR16

Plasmid

#202711

Purpose

integrating bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
URA3 and flanking sequence

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2A-Kina

Plasmid

#202745

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108) : [GGSA]4GG :PIP4K2A (33-406) (PIP4K2A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hMYOD1 gRNA_1-MS2-Puro

Plasmid

#192673

Purpose

Lentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human MYOD1 activating gRNA #1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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OTUB1/pRetroX-IRES-DsRed#10

Plasmid

#180279

Purpose

Plasmid encoding the human protease OTUB1 without a tag. Polycistronic red fluorescent retroviral vector (unmodified from TAKARA BIO).

Depositor

Andre Catic

Article

Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

Insert

human Otubain-1 (OTUB1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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OA-1010Av2

Plasmid

#176082

Purpose

pBac-10xUAS-L596_g9608-p10-UTR-Opie2-dsRed

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

L596_g9608.t1

Use

Tags

3xHA

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1010Bv2

Plasmid

#176083

Purpose

pBac-10xUAS-L596_g25050-p10-UTR-Opie2-dsRed

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

L596_g25050.t1

Use

Tags

3xHA

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-Stx3-L77A/L80A

Plasmid

#100273

Purpose

Expresses GST-Stx3-L77A/L80A in bacteria.

Depositor

Thomas Weimbs

Article

Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

Insert

Stx3-1-265-L77A/L80A (Stx3 Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

No transmembrane domain; residues 4-264 present, …

Promoter

Tac

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-Stx3-E78A/E83A

Plasmid

#100272

Purpose

Expresses GST-Stx3-E78A/E83A in bacteria.

Depositor

Thomas Weimbs

Article

Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

Insert

Stx3-1-265-E78A/E83A (Stx3 Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

No transmembrane domain; residues 4-264 present, …

Promoter

Tac

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-Stx3-LE165/166AA

Plasmid

#100271

Purpose

Expresses GST-tagged open-conformation mutant of Stx3 in bacteria.

Depositor

Thomas Weimbs

Article

Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

Insert

Stx3-1-265-LE165/166AA (Stx3 Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

No transmembrane domain; residues 4-264 present, …

Promoter

tac

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCDB367

Plasmid

#110277

Purpose

gHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusion

Depositor

Christopher Bahl

Article

Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

Insert

gHEEE_02

Use

Tags

10-His, Tev recognition peptide, and mature DsbC …

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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