-
Plasmid#31472DepositorInsertpHRed
UseTags7xHisExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct
Plasmid#176836PurposeTargeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.DepositorInsertdCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
UseUnspecifiedTagsExpressionMutationSee Depositor Comments BelowPromoterTet operator (tetracycline inducible expression)Available sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1_hTRIM32
Plasmid#69541PurposeExpresses human TRIM32 fused to c-terminal EGFPDepositorInserttripartite motif containing 32 (TRIM32 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KO (KanR, 186)
Plasmid#45985DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_XPO1:1-1071
Plasmid#211088PurposeMammalian expression of full-length human XPO1. C-terminal NanoLuc luciferase tag.DepositorInsertXPO1:M1-D1071 (XPO1 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRY2 mCherry Sec61B
Plasmid#102246PurposeExpresses fusion of CRY2PHR with mCherry and Sec61BDepositorInsertCRY2PHR-mCherry-SEC61beta (CRY2 Mustard Weed, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
AcGFP-MSH3
Plasmid#215122PurposeExpresses AcGFP-MSH3 in mammalian cells from a retroviral vector.DepositorInsertMSH3 (MSH3 Human)
UseRetroviralTagsAcGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBA707
Plasmid#186238PurposeKanR-BBR1 entry vector for HR donorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9P35S-N
Plasmid#66191Purposeexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotercauliflower mosaic virus 35S promoter (P35S)Available sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLG011 (DRT type 3)
Plasmid#157889PurposeExpresses the DRT type 3 defense systemDepositorInsertDRT type 3 (RT + RT)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
hMLKL-Venus
Plasmid#106078Purposeexpression in mammalian cellsDepositorInserthuman MLKL-Venus (MLKL Human)
UseRetroviral; Tet-on retrovirusTagsVenusExpressionMammalianMutationPromoterDox-inducibleAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-pro-Il1b-C-Flag
Plasmid#75131PurposeExpresses C-terminal flag-tagged pro-IL-1β in mammalian cellsDepositorInsertinterleukin 1 beta (Il1b Mouse)
UseTags3XFLAGExpressionMammalianMutation(please see depositor comments below)PromoterCMVAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pECFP_C1_NSD2 FL
Plasmid#221382PurposeExpresses NSD2 in mammalian cellsDepositorInsertHistone-lysine N-methyltransferase NSD2 (NSD2 Human)
UseTagsExpressionMammalianMutationL237R mutation in ECFP (Please see depositor comm…PromoterCMVAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA-CBh-eGFP
Plasmid#206885Purposecloning backbone for sgRNA using OptScf2DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterU6Available sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIG-601_NY-ESO-1_TCR_GFP
Plasmid#207482PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertGFP, NY-ESO-1_TCR
UseCRISPRTagsExpressionMutationPotential silent point mutation in GFP sequencePromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-Nb80
Plasmid#205518PurposeEndosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.DepositorInsertendo-Nb80
UseTags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTLR4 flag
Plasmid#13087DepositorInsertTLR4 (Tlr4 Mouse)
UseTagsFlagExpressionMammalianMutationAmino acids 1-19 have been replaced with mammalia…PromoterAvailable sinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-IDH2-R172K-FLAG
Plasmid#66807PurposeTet-inducible expression of mutant IDH2 in mammalian cellsDepositorInsertIDH2 (IDH2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationR172KPromoterCMVAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH2.1_mCherry
Plasmid#205520PurposeExpress mCherry (without export tag)DepositorInsertmCherry
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC19A2
Plasmid#132033PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC19A2 (SLC19A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLG009 (DRT type 1)
Plasmid#157887PurposeExpresses the DRT type 1 defense systemDepositorInsertDRT type 1 (RT-nitrilase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_EGFP
Plasmid#155098PurposeLentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW199b2
Plasmid#136928PurposeDirected evolution of SpCas9DepositorInsertgIII
UseCRISPRTagsExpressionBacterialMutationnonePromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
pHelper_NoMutL_V2_gentR
Plasmid#205293PurposeHelper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.DepositorArticleInsertCspRecT / Bxb-1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-IDH2-FLAG
Plasmid#66806PurposeTet-inducible expression of IDH2 in mammalian cellsDepositorInsertIDH2 (IDH2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_TCF3_PBX1
Plasmid#205937PurposeExpress mEGFP-tagged fusion protein, TCF3_PBX1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CAMK2G
Plasmid#23409DepositorInsertCAMK2G (CAMK2G Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLG012 (DRT type 4)
Plasmid#157890PurposeExpresses the DRT type 4 defense systemDepositorInsertDRT type 4
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG013 (DRT type 5)
Plasmid#157891PurposeExpresses the DRT type 5 defense systemDepositorInsertDRT type 5
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-VEGFR2-N
Plasmid#54298PurposeLocalization: Membrane Receptor, Excitation: 487, Emission: 509DepositorInsertVEGFR2 (KDR Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-CXCL12-sfGFP
Plasmid#98961PurposeMammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12DepositorInsertCXCL12 (CXCL12 Human)
UseTagsSuperfolder GFPExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pLG029 (mzaABCDE)
Plasmid#157907PurposeExpresses the mzaABCDE defense systemDepositorInsertmzaABCDE (5-gene system containing a MutL homolog)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_HaloTag9
Plasmid#169324PurposeExpression of HaloTag9 in bacterial cellsDepositorInsertHaloTag9
UseTagsHisExpressionBacterialMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe EGFR(L858R/T790M)
Plasmid#32073DepositorInsertEGFR (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationL858R/T790MPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-SpG-P2A-hMLH1dn
Plasmid#211550PurposePrime editing in mammalian cellsDepositorInsertPEmax-SpG-P2A-hMLH1dn
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRsetB-His7tag-Peredox-mCherry
Plasmid#32382DepositorInsertHis7-Peredox-mCherry
UseTagsHis7tagExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
Hs.NRAS
Plasmid#83173PurposeGateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions)DepositorInsertNRAS (NRAS Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only