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Plasmid#158667Purposetagging clonal populations with NES mTAGBFP2 fluorescent protein. Adding NES to mTAGBFP2 WCDepositorInsertmTAGBFP2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2 NES visual barcode
Plasmid#158670Purposetagging clonal populations with NES mTurquoise2 fluorescent protein.DepositorInsertmTurquoise2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgGLS_2
Plasmid#163459Purposelentiviral vector expressing Cas9 and an sgRNA targeting GLSDepositorInsertsgRNA 2 targeting GLS (GLS Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
MCP-ADAR2-DD
Plasmid#170124PurposeLentiviral vector carrying the MCP-ADAR2 with a nuclear export signalDepositorInsertMCP-ADAR2-DD-NES
UseLentiviralTagsExpressionMammalianMutationPromoterEf1a core promoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
nuc-mCherry-pTRIP
Plasmid#163520PurposeUsed to construct lentivirus to express nucleus-targeted mCherry in mammalian cellsDepositorInsertnucleus targeted mCherry
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSicoR p53
Plasmid#12090DepositorInsertp53 shRNA (Trp53 Mouse)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationEGFP is expressed from this plasmid as a marker, …PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
TFORF0608
Plasmid#142659PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMAFA (MAFA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T221E
Plasmid#108108PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-Stuffer-HepmCherry
Plasmid#192825PurposeContains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoterDepositorInsertmCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHepatocyte-specific promoter (HS-CRM8-TTRmin modu…Available sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVPRT-tTR-KRAB
Plasmid#11648PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInserthPrion, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLV-Blast_hCOL4A5
Plasmid#198100Purposelentiviral expression of hCOL4A5 in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-PKMYT1_IDG-K
Plasmid#135277PurposeGateway destination clone of PKMYT1 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-PKMYT1 (PKMYT1 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-P2A-BFP
Plasmid#149447PurposeExpresses Cas9 and BFP in a lentiviral vector.DepositorInsertP2A-BFP
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterIn frame with Cas9Available sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-mTagBFP2-2A
Plasmid#102602PurposeControl lentivector. Coexpression of mTagBFP and gene of interest from CMV promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-oct4
Plasmid#20323DepositorInsertoct4 (Pou5f1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgLKB1 clone1
Plasmid#162125PurposeLentiviral sgRNA plasmid targeting human LKB1DepositorInsertsgLKB1 (STK11 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiTet-OTX2-P2A-mCherry BlastR
Plasmid#186741PurposeDox inducible OTX2-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertOTX2 (OTX2 Human)
UseLentiviralTagsP2A-mCherryExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag pLJM1 Rap2A
Plasmid#19311DepositorInsertRAP2A, member of RAS oncogene family (RAP2A Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Neo-EF1A>Tet3G
Plasmid#184379PurposeThe Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)DepositorInsertTet3G
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVXT-mWnt3
Plasmid#121934Purposedoxycycline-inducible mouse Wnt3DepositorInsertWnt3 (Wnt3 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only