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Plasmid#65625PurposeVector for cotranslational expression of tagRFP-Bxb1DepositorInsertBxb1 integrase
UseTagsHA, SV40 NLS, and tagRFPExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-RagC-D181N
Plasmid#42325DepositorInsertRagC D181N (RRAGC Human)
UseTagsFlagExpressionMammalianMutationD181NPromoterCMV, SP6Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-RagB-D163N
Plasmid#42324DepositorInsertRagB D163N (RRAGB Human)
UseTagsFlagExpressionMammalianMutationD163NPromoterCMV, SP6Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUB_smFLAG_ActB_MS2
Plasmid#81083PurposeTranslation reporter: Encodes Beta-actin (374 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).DepositorInsertsmFLAG_ActB_24xMS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL 1903
Plasmid#53020PurposeAmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1DepositorInsertGFP
UseSynthetic BiologyTagsAAV, Asp terminator, and fimSExpressionBacterialMutationPromoterfimSAvailable sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBMN HAYFP-LOV24
Plasmid#49570Purposeregulate protein stability with lightDepositorInsertYFP-LOV24
UseRetroviralTagsHA and YFPExpressionMutationdelete the last 3 aa from AsLOV2, with I532A muta…PromoterLTRAvailable sinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF-TAZ-N-Flag
Plasmid#19025DepositorInsertTAZ (Wwtr1 Mouse)
UseTagsFlagExpressionMammalianMutationVector has no SV40 originPromoterAvailable sinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMDH
Plasmid#133129PurposeA recombinant plasmid expressing mdh (malate dehydrogenase) geneDepositorInsertmalate dehydrogenase gene
UseTagsExpressionBacterialMutationPromotermalate dehydrogenase promoterAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUB_smFLAG_H2B_MS2
Plasmid#81082PurposeTranslation reporter: Encodes Histone H2B (125 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).DepositorInsertsmFLAG_H2B_24xMS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGAL
Plasmid#60337PurposeContains full length native galactose promoterDepositorInsertspromoter
yECitrine
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCK01_PalkS_alkS
Plasmid#166502PurposeExpression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertalkS
UseSynthetic BiologyTagsExpressionBacterialMutationL401GPromoterPalkSAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI
Plasmid#116955PurposeExpresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOBCol2.3-GFPemd
Plasmid#110210PurposeLabels cells in the late osteoblast lineage.DepositorInserteGFPemerald
UseTagsNoneExpressionMammalianMutationPromoter2.3 kb rat type 1 collagen + first intronAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWJB30
Plasmid#126505PurposeSalmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30DepositorInsertadrA-8xHis
UseTags8xHisExpressionBacterialMutationPromoterPBAD30Available sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMZ1001
Plasmid#204398PurposeA pC-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PRpa0002Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ3012
Plasmid#204400PurposeA C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PRhl0L0L12Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Luc-SIRT1 mutant 3'UTR
Plasmid#20380DepositorInsertSIRT1 (SIRT1 Human)
UseLuciferaseTagsExpressionMutationmutant 3'UTR of SIRT1PromoterAvailable sinceMay 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMK401 (Hygro-P2A-mAID-Nluc)
Plasmid#121196PurposeN-terminal taggingDepositorInsertHygro-P2A-mAID-Nluc
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV MN (eGFP)
Plasmid#89932PurposeExpresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.DepositorInsertMN
UseTagsHAExpressionMammalianMutationM.SssI residues 2-272 fragmentPromoterCMVAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-pro-sumo2
Plasmid#25102DepositorInsertpro-SUMO2 (SUMO2 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only