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p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

Plasmid

#158585

Purpose

Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

membrane localization signal-tagBFP-wrmScarlet11

Use

Tags

tagBFP

Expression

Yeast

Mutation

GGGS linker

Promoter

TEF

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-Cln6

Plasmid

#78109

Purpose

mammalian expression of mouse Cln6 with C terminal EGFP fusion

Depositor

Thomas Braulke

Article

Thelen et al PLoS One. 2012;7(4):e35493. doi: 10.1371

Insert

Cln6 (Cln6 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only
tdEos-Farnesyl-5

Plasmid

#57613

Purpose

Localization: Plasma Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Farnesyl (HRAS Human)

Use

Tags

tdEos

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCSJ982

Plasmid

#176622

Purpose

Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

Depositor

Alexander Varshavsky

Article

Dong et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

Insert

Ub-MPGLW-Fbp1

Use

Tags

Ubiquitin N-terminal fusion and triple flag tag C…

Expression

Yeast

Mutation

Changed first 4 N-terminal amino acid residues of…

Promoter

TDH3tc3

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pmApple-EGFP-HRas

Plasmid

#133854

Purpose

Mammalian expression of HRas N-terminally tagged with mApple and EGFP

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

HRas (HRAS Human)

Use

Tags

EGFP and mApple

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSE360-Prp22

Plasmid

#111284

Purpose

Prp22 WT gene in pSE360 backbone

Depositor

Christine Guthrie

Article

Noble et al Genetics. 1996 May;143(1):67-80.

Insert

PRP22

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Sub2 pRS315

Plasmid

#111300

Purpose

Sub2 in pRS315 vector

Depositor

Christine Guthrie

Article

Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

Insert

SUB2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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yCp50-PRP2

Plasmid

#111304

Purpose

Prp2 in yCp50 plasmid

Depositor

Christine Guthrie

Article

Noble et al Genetics. 1996 May;143(1):67-80.

Insert

PRP2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pDE43-MCKq20

Plasmid

#27334

Depositor

Dirk Schnappinger

Article

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

qtag20

Use

Gateway destination vector

Tags

Expression

Mutation

N/A

Promoter

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pDE43-MCKq19

Plasmid

#27286

Depositor

Dirk Schnappinger

Article

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

qtag19

Use

Gateway destination vector

Tags

Expression

Mutation

N/A

Promoter

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pDE43-MCKq26

Plasmid

#27336

Depositor

Dirk Schnappinger

Article

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

qtag26

Use

Gateway destination vector

Tags

Expression

Mutation

N/A

Promoter

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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yCp50-PRP5

Plasmid

#111303

Purpose

Prp5 in yCp50 plasmid

Depositor

Christine Guthrie

Article

Noble et al Genetics. 1996 May;143(1):67-80.

Insert

PRP5

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMCB320-sgNC.SPA

Plasmid

#169834

Purpose

Expresses a negative control sgRNA

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

safe harbor sgRNA

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ166-SpRY

Plasmid

#161520

Purpose

Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter

Depositor

Yiping Qi

Article

Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

Insert

zSpRYCas9

Use

CRISPR; Gateway compatible zsprycas9 entry clone

Tags

3X FLAG, NLS and NLS

Expression

Plant

Mutation

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ262-ABE8e

Plasmid

#161523

Purpose

Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editing

Depositor

Yiping Qi

Article

Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

Insert

ABE8e-zSpCas9(D10A)

Use

CRISPR; Gateway compatible abe8e-zspcas9(d10a) en…

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_eGFP (open)

Plasmid

#160094

Purpose

Expresses eGFP in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

eGFP

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ262B-ABE8e

Plasmid

#161524

Purpose

Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing

Depositor

Yiping Qi

Article

Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

Insert

TadA8e-zSpRYCas9(D10A)

Use

CRISPR; Gateway compatible tada8e-zsprycas9(d10a)…

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgNCKAP1-2

Plasmid

#210134

Purpose

knock out NCKAP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Nck-associated protein 1 (NCKAP1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgNCKAP1-1

Plasmid

#210133

Purpose

knock out NCKAP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Nck-associated protein 1 (NCKAP1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTarget-RFP-GFP operon

Plasmid

#192279

Purpose

RFP GFP fusion protein expressed under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

RFP GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacIq

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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