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Plasmid#177014PurposeGenerate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEVDepositorInsertCAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
UseTagsPolyhistidine-TEVExpressionInsectMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-2xPH-TAPP1 WT
Plasmid#161990PurposeExpresses two tandem repeats of Tapp1 PH domain that binds to PI(3,4)P2. Fused to eGFPDepositorInsertPH-TAPP1 (PLEKHA1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-I_peroxisome_C1
Plasmid#85065PurposeIn vivo visualization of the peroxisome (can be used for colocalization studies)DepositorInsertPeroxisomal targeting sequence
UseTagsmScarlet-iExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_17G6-1 heavy chain
Plasmid#198249Purposerecombinant expression of heavy chain of monoclonal anti-AMP antibody 17G6-1DepositorInsertheavy chain of monoclonal anti-AMP antibody 17G6-1 (mouse IgG2b)
UseTagsIL10 signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSR657
Plasmid#65214PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-2 cap
UseTagsExpressionInsectMutationPromoterp10 and polyhedrinAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam N term His8 eGFP 3C
Plasmid#160684PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneUseTagsHis8 eGFP 3CExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS MCK promoter
Plasmid#12528DepositorInsertMCK promoter (Ckm Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-ATP6V0D1
Plasmid#87931PurposeFLAG-tagged overexpressionDepositorInsertATP6Vod1 (ATP6V0D1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI HA NEDD4L
Plasmid#27000DepositorInsertNEDD4L (NEDD4L Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462)
Plasmid#48141PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62987DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSR660
Plasmid#65216PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-8 cap
UseTagsExpressionInsectMutationPromoterP10 and polyhedrinAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
TRE-VEGFA EF1a-rtTA3
Plasmid#195881PurposeLentiviral construct of doxycycline inducible VEGFADepositorInsertVEGFA (Vegfa Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Jmjd3-F
Plasmid#17440PurposeExpression of Jmjd3 in mammalian cellsDepositorInsertJMJD3 (Kdm6b Mouse)
UseTags6xMyc and FlagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP.90 beta
Plasmid#108221PurposeMammalian expression vector for EGFP fusion to human Hsp90 betaDepositorInsertEGFP-Hsp90b (HSP90AB1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1a_PAX7_P2A_Hygro_Barcode
Plasmid#120472PurposeBarcoded lentiviral vector to express PAX7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertPAX7 (PAX7 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-Nb80
Plasmid#205518PurposeEndosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.DepositorInsertendo-Nb80
UseTags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hKLF4
Plasmid#17219PurposeRetroviral expression of human KLF4DepositorInsertKLF4 (KLF4 Human)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
HA-Foxo1 (pCMV5)
Plasmid#12142DepositorInsertFoxo1 (Foxo1 Mouse)
UseTagsHA and MycExpressionMammalianMutationWild-typePromoterCMVAvailable sinceAug. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS/ES-EnvA-VSVg-WPRE-pA
Plasmid#87221Purposeto package wild type EnvA psudotyped lentivirusDepositorInsertEnvA-VSVg
UseMouse Targeting and Synthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only