Addgene

We narrowed to 34,675 results for: KAN

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pBABE-hygro NM23 H1 His

Plasmid

#11365

Depositor

Ronald Kahn

Article

Zhu et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

Insert

NM23 H1 (NME1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

His118 mutation to Phe (abolishes histidine kinas…

Promoter

Available since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
Lyn-CFP-GAI(1-151)

Plasmid

#37312

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Use

Tags

CFP and Lyn

Expression

Mammalian

Mutation

contains aa 1-151

Promoter

CMV

Available since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
pJ02B2Gm_AE

Plasmid

#66063

Purpose

MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB222

Plasmid

#68613

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB219

Plasmid

#68610

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pet23a_RBPMS_1_101

Plasmid

#65728

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-101

Promoter

T7

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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8200 Bicistronic_GFP_ires_hygro

Plasmid

#64375

Purpose

This a retroviral expression plasmid expressing GFP along with hygro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

EGFP

Use

Retroviral

Tags

EGFP

Expression

Mutation

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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Lyn-CFP-GAI(1-532)

Plasmid

#37314

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Use

Tags

CFP and Lyn

Expression

Mammalian

Mutation

contains aa 1-532

Promoter

CMV

Available since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
pOPTXcGMPRELUC

Plasmid

#68503

Purpose

cyclic nucleotide reporter for bacteria or plant cells

Depositor

Helen Irving

Article

Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

Insert

OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)

Use

Tags

luciferase

Expression

Bacterial and Plant

Mutation

3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

Promoter

OPTX promoter with cGMPRE

Available since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only
Nuc-Orange Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71349

Purpose

Express Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern(Ca2+)_H2B

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
pGAL4-DBD-RUNX2+10A-IDR

Plasmid

#145251

Purpose

Expresses fusion of GAL4 DNA-binding domain and RUNX2A+10A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2A+10A-IDR

Use

Tags

Expression

Mammalian

Mutation

A-repeat expanded

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJ02B2Gm_EF

Plasmid

#66064

Purpose

MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB10

Plasmid

#63111

Purpose

Expression of HSPB10 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb10 (ODF1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pMpGWB319

Plasmid

#68647

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB221

Plasmid

#68612

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Mutation

Promoter

MpEF1alpha promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pTV-mHdac1-tdiRFP670-PEST2

Plasmid

#122351

Purpose

Targeting vector for mouse Hdac1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Hdac1

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET23a_mod_RBPMS2_FL

Plasmid

#59394

Purpose

Plasmid for bacterial overexpression of RBPMS2

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS2 (RBPMS2 Human)

Use

Tags

6xHis, FLAG, HA, and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pTV-Met-Eif4a1-tdiRFP670-PEST

Plasmid

#122371

Purpose

Targeting vector for mouse Eif4a1 to insert 2A-tdiRFP670-PEST-2A into immediately downstream of start codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST-2A with homology arms for Eif4a1

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Eif4a1-L

Plasmid

#122346

Purpose

Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Eif4a1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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6691 Bicistronic_ires_puro

Plasmid

#64335

Purpose

This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge