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Plasmid#195840PurposeExpresses human FZD2 with an N-terminal HiBiTDepositorInsertFZD2 (FZD2 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH-nCas9-PPE
Plasmid#140447PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertnCas9(H840A)-M-MLV
UseCRISPRTagsExpressionPlantMutationH840A for Cas9; D200N, T306K, W313F, T330P and L…Promotermaize Ubiquitin-1, OsU3Available sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET19-MTdTc302
Plasmid#207458PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302DepositorInsertMTdTc302 (Dntt Mouse)
UseTags10xHis-MBPExpressionBacterialMutationCys188Ala, Cys216Ser, Cys378Ala, and Cys438SerPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SUMO)10-(SIM)6
Plasmid#122026PurposeMammalian expression plasmid for multivalent scaffold of (SUMO)10-(SIM)6 biomolecular condensates to recruit SIM-containing client proteins.DepositorInsertUseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ 2xFLAG-2xSTREP_FBXO22
Plasmid#159127Purposeexpresses FBXO22 in mammalian cellsDepositorInsertFBXO22 (FBXO22 Human)
UseTags2X-FLAG _ 2X-STREPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pROF141
Plasmid#155329PurposeBinary vector for expression of the PULSE system under the control of PAtUbi10 promoterDepositorInsertLB_PAtUbi10-SRDX-NLS-EL222-Tnos_SF_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF402
Plasmid#155334PurposeBinary vector for expression of FLuc controlled by the blue light-repressible systemDepositorInsertLB_P35Senhancer(-953 to -51)-(C120)5-PhCMVmin-FLuc-T35S_RB
UseLuciferase and Synthetic Biology; Eukaryotic expr…TagsExpressionMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF441
Plasmid#155336PurposeBinary vector for expression of a gRNA targeting AtAP1 promoterDepositorInsertLB_PAtU6-26-gRNA(PAtAP1)-sgRNA_RB
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKD1
Plasmid#170846PurposeAAV backbone for transgene expression in cortical interneurons under the control of the Dlx enhancer.DepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SUMO)6-(SIM)10
Plasmid#122027PurposeMammalian expression plasmid for multivalent scaffold of (SUMO)6-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.DepositorInsertUseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-D672G-GFP
Plasmid#78919PurposeMammalian expression of BAP1 with D672G and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationD672GPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-F81V-GFP
Plasmid#78912PurposeMammalian expression of BAP1 with F81V and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationF81VPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-mCherry-p53 deltaN
Plasmid#49243Purposeexpresses human p53 deltaN and mCherry in mammalian cellsDepositorInsertp53 deltaN (TP53 Human)
UseTagsIRES-mCherryExpressionMammalianMutationdeltaN ( lacks 39 residues at the N-terminus)PromoterCMVAvailable sinceDec. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
2xmyc-JIP4
Plasmid#164633PurposeExpresses JIP4 in mammalian cells with a 2xmyc tagDepositorInsertJIP4 (SPAG9 Human)
UseTags2xmycExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCK301
Plasmid#87767PurposeE. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacIDepositorInsertPrhaBAD-sfGFP
UseSynthetic BiologyTags6xHis TagExpressionBacterialMutationPromoterPrhaBAD rhamnose-inducible promoter from E. coliAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-H169Q-GFP
Plasmid#78917PurposeMammalian expression of BAP1 with H169Q and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationH169QPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-C91G-GFP
Plasmid#78916PurposeMammalian expression of BAP1 with C91G and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationC91GPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX304-YAP1 _TA
Plasmid#59143PurposeYAP1 mutants that harbor a deletion of the transcriptional activation domainDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationTA (transactivation) domain deleted; aa291-497PromoterCMVAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
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HiBiT-FZD9
Plasmid#195847PurposeExpresses human FZD9 with an N-terminal HiBiT; contains P199R; I448V; S524N; R556HDepositorInsertFZD9 (FZD9 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-sCre
Plasmid#55635PurposeExpresses sCre in Mammalian CellsDepositorInsertCre
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-RGS7
Plasmid#55760PurposeAn amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.DepositorInsertmCer(1-158)-RGS7 (RGS7 Aequorea victoria, Human)
UseTagsCer(1-158) was fused to the amino terminus of RGS…ExpressionMammalianMutationRGS7 was amplified via PCR, which added an N-term…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p323.hSyn.Halo-mFMRP.Wpre
Plasmid#185909PurposeExpresses FMRP fused to HaloTag for protein localization through fluorescence imagingDepositorInsertHaloTag fused to mouse Fragile X mental retardation protein (Ctsa Mouse)
UseLentiviralTagsHaloTagExpressionMutationPromoterhSyn (human Synapsin promoter)Available sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.SV40.THBS4-HA.SV40(polyA)
Plasmid#155193PurposeExpresses murine Thrombospondin-4 (THBS4) protein with HA-tag at C-terminusDepositorInsertThrombospondin-4 (Thbs4 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS1
Plasmid#170852PurposeAAV backbone for transgene expression in neurons under the control of the hSyn1 promoter.DepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
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nCas9-PPE
Plasmid#140445PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertnCas9(H840A)-M-MLV
UseCRISPRTagsExpressionPlantMutationH840A for Cas9; D200N, T306K, W313F, T330P and L…Promotermaize Ubiquitin-1Available sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-mCherryTRF1-IRES-Blast
Plasmid#182588Purposeexpresses mcherry-trf1 in mammalian cellsDepositorInserthTRF1 (TERF1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF-1aAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PuroDTK.Neo
Plasmid#72307PurposePiggyBac Cassette contains puromycin and TK genesDepositorInsertPuromycin-DTK-neo
UseTagsExpressionBacterial and MammalianMutationPromoterCAG promoterAvailable sinceFeb. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-2xTS
Plasmid#109419PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX304-YAP1 _PDZ
Plasmid#59147PurposeYAP1 mutants that harbored a deletion of the 5 amino acidsDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMammalianMutationPDZ binding domain (last 5aa) deletedPromoterCMVAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-Ubl
Plasmid#78712PurposeExpresses Sacsin Ubl- domain (2-85) in fusion with GST (bacterial)DepositorInsertHuman Sacsin 2-85
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cry2 mCherry GRK2 ct in pcDNA3.1
Plasmid#64212PurposemCherry fused between Cry2 and GRK2ct to study cell migrationDepositorUseTagsmcherryExpressionMammalianMutationPromoterCMV and cmvAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-sr1(SIRPT1)
Plasmid#75297PurposeExpresses Sacsin sr1 (SIRPT1 repeat) domain (84-339) in fusion with GST (bacterial)DepositorInsertHuman Sacsin 84-339
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Ascl1
Plasmid#134671PurposeRetroviral expression of mouse Ascl1DepositorInsertAscl1 (Ascl1 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-Ubl-sr1(SIRPT1)
Plasmid#75298PurposeExpresses Sacsin Ubl-sr1 (SIRPT1 repeat) domain (1-339) in fusion with GST (bacterial)DepositorInsertHuman Sacsin 1-339
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
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Cry2 mCherry GRK3 ct in pcDNA3.1
Plasmid#64213PurposemCherry fused between Cry2 and GRK3 ct to study cell migrationDepositorUseTagsmcherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
GAL-hAR-658-919
Plasmid#89082Purposemammalian expression of human androgen receptor ligand binding domain 658-919 amino acids linked to GAL4 DNA binding domainDepositorInserthAR-658-919 (AR Human)
UseTagsGAL4 DBDExpressionMammalianMutationaa 658-919PromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR-53BP1 1-1972 WT
Plasmid#53446PurposeRecombinational donor/master vectorDepositorInsert53BP1 (TP53BP1 Human)
UseGatewayTagsExpressionMutationsiRNA resistant and mutations A128V, T720A, A1347…PromoterAvailable sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS-mNG-eDHFR(69K6)-G-alpha-q
Plasmid#172106PurposeMammalian expression of Gαq fused to mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)-Gαq (GNAQ Human)
UseTagsExpressionMammalianMutationGαq: C9S/C10S/Q209LPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-hAR-663-919
Plasmid#89083Purposebacterial expression of human androgen receptor ligand bindng domain amino acid residues 663-919 with histidine tagDepositorInserthAR ligand binding domain (AR Human)
UseTagsHisExpressionBacterialMutationaa 663-919PromoterT7Available sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2
Plasmid#190898PurposeEntry vector for multiple sgRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6 and 7skAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-ETV6
Plasmid#131594Purposedoxycycline-inducible expression of human ETV6 in mammalian cellsDepositorInsertETV6 (ETV6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-mCMVAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET19-M(BRCT)TdTwt
Plasmid#207459PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domainDepositorInsertM(BRCT)TdTwt (Dntt Mouse)
UseTags10xHis-MBPExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Neo-PuroDTK
Plasmid#84036PurposePiggyBac cassette contains Neomycin, puromycin and TK genesDepositorTypeEmpty backboneUseTagsExpressionBacterial and MammalianMutationPromoterCAG promoterAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
KA1042_pPB-hCMV*1-Esrrb-IV
Plasmid#124177PurposepiggyBac-based Dox-inducible expression of EsrrbDepositorInsertEsrrb-IRES-Venus (Esrrb Mouse)
UseTagsExpressionMammalianMutationPromotertetO-CMVminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Nrf1-Myc pcDNA3.1(+)-C-Myc (NAWLVH106-AAAAAA)
Plasmid#169126Purposeexpression of proteinDepositorInserthNrf1 (NFE2L1 Human)
UseTagsExpressionMammalianMutationNAWLVH106-AAAAAAPromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
mIFP12-sEspin-C-18
Plasmid#56262PurposeLocalization: Actin Bundling, Excitation: 683, Emission: 704DepositorInsertsEspin
UseTagsmIFP12ExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
LUC2 (L/Sec/deltaP)
Plasmid#112145PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
UseTagsExpressionMammalianMutationCysteine 258 mutated to selenocysteine (U) and d…PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only