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Showing: 11901 - 11950 of 148149 results

  6. pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

    Plasmid
    #218508
    Purpose
    integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mNeongreen
    Use
    Tags
    modified ssrA degradation tag
    Expression
    Bacterial
    Mutation
    deletion of MCS containing Plac_lacZa, introducti…
    Promoter
    P23
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218508-plasmid-map-sequence-id-438746

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  7. pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

    Plasmid
    #218509
    Purpose
    integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mKate2
    Use
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Deletion of Plac_mrfp cassette, introduction of a…
    Promoter
    P23
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218509-plasmid-map-sequence-id-438730

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  16. pMax_mPlum_C207G/T208C

    Plasmid
    #177831
    Purpose
    Site-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).
    Depositor
    Zachary Nagel, Leona Samson
    Article
    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111
    Insert
    mPlum_C207G/T208C
    Use
    Tags
    Expression
    Mammalian
    Mutation
    C207G + T208C
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177831-plasmid-map-sequence-id-348956

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  20. pV1093

    Plasmid
    #111428
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111428-plasmid-map-sequence-id-212463

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  21. pCW57.1_SPTLC1_C133W

    Plasmid
    #181921
    Purpose
    3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133W
    Depositor
    Christian Metallo
    Article
    Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10
    Insert
    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)
    Use
    Lentiviral; Mammalian expression, , doxycycline i…
    Tags
    Expression
    Mammalian
    Mutation
    cysteine at position 133 of the coding region of …
    Promoter
    tight TRE promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    181921-plasmid-map-sequence-id-361675

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  22. pLB

    Plasmid
    #11619
    Purpose
    pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.
    Depositor
    Stephan Kissler
    Article
    Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6
    Available since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11619-plasmid-map-sequence-id-274493

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  25. pmROSA26-attP50-Neo-mKate2-3xNLS-attP50

    Plasmid
    #183609
    Purpose
    Targeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Inserts
    Neo
    mKate2-3xNLS
    Use
    Mouse Targeting
    Tags
    3xNLS
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183609-plasmid-map-sequence-id-361597

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  29. BcatMutS33/S37.T41/S45

    Plasmid
    #24204
    Depositor
    David Rimm
    Insert
    beta catenin (CTNNB1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    S33A, S37A, T41A,S45A
    Promoter
    CMV
    Available since
    March 9, 2010
    Availability
    Academic Institutions and Nonprofits only
    24204-plasmid-map-sequence-id-235877

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  31. pBABE-MCS(extended)-Puro (empty vector)

    Plasmid
    #172603
    Purpose
    Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    The multiple cloning site (MCS) has been edited/e…
    Promoter
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172603-plasmid-map-sequence-id-340216

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  34. pTRE3G-VP64-dCas9-VP64-ZsG

    Plasmid
    #192668
    Purpose
    Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    VP64-dCas9-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    TRE3G
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192668-plasmid-map-sequence-id-379892

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  36. pTCF156 HLA-A*02:01

    Plasmid
    #180456
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*02:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180456-plasmid-map-sequence-id-355799

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  45. pBA950

    Plasmid
    #122239
    Purpose
    CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122239-plasmid-map-sequence-id-292012

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Showing: 11901 - 11950 of 148149 results