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pCEP4-myc-PDGFRbeta

Plasmid

#136455

Purpose

Mammalian expression plasmid for c-myc-tagged PDGFRB

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

PDGFRbeta (PDGFRB Human)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-trGluc

Plasmid

#158959

Purpose

DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

trGluc

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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AICSDP-25:MAP1LC3B-mEGFP

Plasmid

#101783

Purpose

Homology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3B

Depositor

Allen Institute for Cell Science

Insert

MAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

Oct. 31, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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AAV_Actb HR donor

Plasmid

#97317

Purpose

HR donor for fusing a p2A-mCherry reporter to mouse Actb.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HR donor

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pKan-PCUP1-9myc-AID*(N)

Plasmid

#99527

Purpose

N-terminal 9myc-AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-117) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Expression

Mutation

Promoter

CUP1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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p3015b

Plasmid

#209317

Purpose

Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

Depositor

Thomas Hooven

Article

Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

Insert

Group B Streptococcus-compatible single guide RNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Xyl/tet promoter that is constitutively active

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLX317-hcRED

Plasmid

#115440

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

hcRED

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shLacZ-1735

Plasmid

#115454

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shLacZ-1735

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pKan-PCUP1-AID*(N)-9myc

Plasmid

#99526

Purpose

N-terminal AID*-9myc degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Expression

Mutation

Promoter

CUP1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHDR_NANOS3-T2A-mVenus-PuroTK

Plasmid

#222903

Purpose

Homology directed repair template for knocking in mVenus reporter to NANOS3.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mVenus

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP

Plasmid

#139485

Purpose

PCR template for Halo-Tag gene knock-in donor vector

Depositor

Kazuhiro Aoki

Article

Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

Insert

HaloTag-loxP-P2A-dTKneo-pA-loxP

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)

Plasmid

#191647

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-GAPDH-IRES-EGFP

Plasmid

#115918

Purpose

donor to insert IRES-EGFP at the GAPDH locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

IRES-EGFP

Use

Donor template

Tags

dTomato

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEasyG1_hph

Plasmid

#184912

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-ACTB-mEGFP

Plasmid

#115917

Purpose

donor to insert EGFP at the ACTB locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

ACTB-mEGFP

Use

Donor template

Tags

EGFP

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)

Plasmid

#191635

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBCMV-HaloTag-VPg(FCV)

Plasmid

#167311

Purpose

Template pDNA to prepare a split CaVT component mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

HaloTag-VPg(FCV)

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-TOMM20-mEGFP

Plasmid

#115923

Purpose

donor to insert EGFP at the TOMM20 locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

TOMM20-mEGFP

Use

Donor template

Tags

EGFP

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEasyG1_nat

Plasmid

#184911

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-mHopX

Plasmid

#16425

Depositor

Jonathan Epstein

Article

Chen et al Cell. 2002 Sep 20. 110(6):713-23.

Insert

Hopx (hop Mouse)

Use

In situ template

Tags

Expression

Mutation

None

Promoter

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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