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Plasmid#110734PurposeRecombination template for replacing CDKN2A Exon2 with CMV-EGFPDepositorInsertEGFP
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_10E
Plasmid#91209PurposeDirect Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers , Plant Selection: noneDepositorInsert35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
EF.deltaBHES1.Ubc.GFP
Plasmid#24982DepositorInsertHES1 (HES1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationDNA-binding domain disrupted by E43A, K44A, R47A …PromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-L-R279E
Plasmid#182286PurposeMammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx50-20xMS2-Hyg
Plasmid#210219PurposeIntegrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
UseTagsExpressionMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSMOX-mNG::LacI-V3
Plasmid#210224PurposeIntegrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.DepositorInsertmNeongreen Lac repressor fusion protein
UseTrypanosoma brucei expressionTagsExpressionMutationPromoterPol II readthrough transcription after integrationAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pdCASclos
Plasmid#73640PurposeTranscriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824DepositorInsertsdCas9
sgRNA to spo0A
UseE.coli-clostridium shuttle vectorTagsExpressionMutationD10A, H840APromoterPj23119 and ptbAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
MMLV-RT for Split-PE (pEH45)
Plasmid#190105PurposeExpresses MMLV-RT (full-length, from PE2) for Split-PE useDepositorInsertMMLV-RT
UseTagsbpNLSExpressionMammalianMutationRT pentamutant from PE2 (D200N, T306K, W313F, T33…PromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP91
Plasmid#83548PurposeUsed to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.DepositorInsertPGK1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterPGK1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
3540 pMIG Bid p15
Plasmid#8789DepositorInsertBid p15 (Bid Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGL3-B-pGAS5
Plasmid#46371DepositorInsertGAS5 (GAS5 Human)
UseLuciferaseTagsluciferaseExpressionMutationpromoterPromoterAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
3538 pMIG Bad
Plasmid#8787DepositorInsertBad (Bad Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAsCpf1(TYCV)(BB) (pY211)
Plasmid#89352PurposeExpresses humanized AsCpf1 TYCV PAM variant and crRNA guideDepositorInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K607RPromoterCBhAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pILGFP92
Plasmid#83549PurposeUsed to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.DepositorInsertTDH3 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTDH3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH (Merlin)
Plasmid#136448PurposeMammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-lengthDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro SCRIB WT
Plasmid#88886PurposeSCRIB WT in MSCV backboneDepositorInsertSCRIB (SCRIB Human)
UseRetroviralTagsN/AExpressionMutationN/APromoterMSCV promoterAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
ires-eGFP(+HAs) donor-1
Plasmid#87865PurposeExpress eGFP reporter for HDR-induced knock-inDepositorInserteGFP
UseHomologous recombination for gapdh lociTagsExpressionMutationPromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd36
Plasmid#98302PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.DepositorInsertPCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pILGFPB5A
Plasmid#83556PurposeUsed to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.DepositorInsertGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL1Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SP-CLSTN2-3xHAtag
Plasmid#191693PurposeFor expression coding sequencing of CLSTN2DepositorInsertsignal peptide, full length coding sequence of CLSTN2 and 3xHAtag
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only