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Plasmid#99654PurposeExpresses dSp Cas9 fused to p65 activation domainDepositorArticleInsertdCas9
UseTagsp65 activation domainExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-p65 (150-261)
Plasmid#99655PurposeExpresses dSp Cas9 fused to truncated p65 activation domainDepositorArticleInsertdCas9
UseTagstruncated p65 activation domain (150-261)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-p65 (100-261)
Plasmid#99656PurposeExpresses dSp Cas9 fused to truncated p65 activation domainDepositorArticleInsertdCas9
UseTagstruncated p65 activation domain (100-261)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-p65 (200-261)
Plasmid#99657PurposeExpresses dSp Cas9 fused to truncated p65 activation domainDepositorArticleInsertdCas9
UseTagstruncated p65 activation domain (200-261)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-p65 (1-200)
Plasmid#99658PurposeExpresses dSp Cas9 fused to truncated p65 activation domainDepositorArticleInsertdCas9
UseTagstruncated p65 activation domain (1-200)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-p65 (1-150)
Plasmid#99659PurposeExpresses dSp Cas9 fused to truncated p65 activation domainDepositorArticleInsertdCas9
UseTagstruncated p65 activation domain (1-150)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMCV
Plasmid#199743PurposeMinimal cloning vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
dSp-Rta 100-175
Plasmid#99667PurposeExpresses dSp Cas9 fused to truncated RTA activation domainDepositorArticleInsertdCas9
UseTagstruncated RTA activation domain (101-175)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
dSp-Rta 50-175
Plasmid#99665PurposeExpresses dSp Cas9 fused to truncated RTA activation domainDepositorArticleInsertdCas9
UseTagstruncated RTA activation domain (51-175)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pErCas12a EZ Clone
Plasmid#132641PurposeExpresses ErCas12a and an sgRNA to target a region of interestDepositorInsertErCas12a
UseAAV and CRISPRTags3x HA and SV40 NLSExpressionMammalianMutationQ926R (Please see depositor comments) and BsaI si…PromoterCMV PromoterAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Rosa26-twinPE-attB
Plasmid#207859PurposeAAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Rosa26 twinPE pegRNA pairsDepositorInsertNpuC-Cterm-PEmaxDRNaseH-dualU6-Rosa26-twinPE-attB
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Dnmt1-PE3-loxP
Plasmid#207862PurposeAAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNADepositorInsertNpuC-Cterm-PEmaxDRNaseH-dualU6-Dnmt1-PE3-loxP
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX551-miniCMV-SpCas9D10A nickase
Plasmid#216735PurposeExpresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)DepositorArticleInsertCas9-D10A nickase
UseAAV and CRISPRTagsExpressionMammalianMutationD10APromoterT7 and mini-CMVAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
N-Terminal Split Cas9 D10A Nickase with GyrA intein
Plasmid#58695PurposeExpresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase productionDepositorInsertD10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
UseAAV and CRISPRTagsGyrA Nsplit Intein, HA Tag, and NLSExpressionMammalianMutationD10A nickase converting mutation to SpCas9PromoterCBhAvailable sinceSept. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEMS2144
Plasmid#104357PurposeAAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporterDepositorInsertssAAV-smCBA-EmGFP
UseAAVTagsExpressionMutationPromotersmCBAAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
hSyn-GtACR2-ChRmine-tandem-eYFP
Plasmid#192580PurposeAAV-vector for bidirectional optogenetic manipulation of neuronsDepositorInsertsomBiPOLES
UseAAVTagsEYFP- soma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterhuman synapsinAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-crRNA(TARDBP)
Plasmid#109323PurposeAAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TARDBPDepositorInsertmCherry-KASH (TARDBP Human)
UseAAVTagsExpressionMutationPromoterhSyn1Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-crRNA(SOD1)
Plasmid#109321PurposeAAV vector expressing crRNA for AsCpf1 (RR variant) targeting human SOD1DepositorInsertmCherry-KASH (SOD1 Human)
UseAAVTagsExpressionMutationPromoterhSyn1Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
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