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Plasmid#124292PurposeExpression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.DepositorInsertvsfGFP-9
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-Nb37
Plasmid#205514PurposeEndosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.DepositorInsertendo-Nb37
UseTags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-GFP (PX461)
Plasmid#48140PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorInserthSpCas9n
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
mRFP-FKBP12
Plasmid#67514PurposeFKBP12 onlyDepositorInsertFKBP12 (FKBP1A Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-OTUB1 WT-pcDNA3.1
Plasmid#118209PurposeExpresses FLAG-OTUB1 WT in mammalian cellsDepositorInsertOTUB1 (OTUB1 Human)
UseTags3x FLAGExpressionMammalianMutationnonePromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGM51323
Plasmid#153224PurposeLevel 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminatorDepositorInsertArabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM35171
Plasmid#153222PurposeLevel 1 position 1 part containing the Nos promoter, the BAR gene, and the Ocs terminatorDepositorInsertNos promoter, BAR gene, Ocs terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICH67131
Plasmid#153223PurposeLevel 1 position 1 containing the Nos promoter, NPTII (Kan resistance), and the Nos terminatorDepositorInsertNos promoter, NPTII, Nos terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7
Plasmid#121944PurposeCRISPR-Sirius plasmidDepositorInserthU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6 promotersAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T MDM2 WT
Plasmid#16237DepositorInsertMDM2 (MDM2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLV_hEF1a_rtTA3
Plasmid#61472Purpose3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)DepositorInsertrtTA3
UseLentiviralTagsExpressionMutationPromoterhEF1aAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBBR_Cas9_sp1
Plasmid#154851PurposeExpresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genomeDepositorInsertsSpcas9 gene
sgRNA scaffold
UseCRISPRTags6xHisExpressionMutationCodon harmonized variant for expression in Rhodob…PromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1_Puro
Plasmid#125594PurposeLentiviral expression of sgRNA with GFP and puromycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for sgRNA expression and EFS promoter…Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 MDM2 S166D S186D
Plasmid#16236DepositorInsertMDM2 (MDM2 Human)
UseTagsExpressionMammalianMutationSer166 and Ser186 replaced by AspPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL4 mIL-17 0.6kb promoter
Plasmid#20126DepositorInsertmIL-17 (Il17a Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 MDM2 S166D
Plasmid#16234DepositorInsertMDM2 (MDM2 Human)
UseTagsExpressionMammalianMutationSer166 replaced by AspPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 MDM2 S186D
Plasmid#16235DepositorInsertMDM2 (MDM2 Human)
UseTagsExpressionMammalianMutationSer186 replaced by AspPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3
Plasmid#90087PurposeExpresses wild type caspase 3 in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationWild typePromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFDH
Plasmid#131706PurposeExpress constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coliDepositorInsertformate dehydrogenase
UseTagshis-tagExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-ARID1A
Plasmid#39311DepositorInsertARID1A (ARID1A Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only